Walle T K, Vartio T, Helve T, Virtanen I, Kurki P
Department of Anatomy, University of Helsinki, Finland.
Scand J Immunol. 1990 Apr;31(4):535-40. doi: 10.1111/j.1365-3083.1990.tb02802.x.
Mouse monoclonal antibodies against ED sequence-containing cellular fibronectin (cFn) were used to show that Fn in the inflamed synovium is distinct from the major form of plasma Fn (pFn). An accumulation of cFn was seen at sites of hyperplasia of the rheumatoid synovial membrane and in the walls of small vessels in the synovium by immunofluorescence microscopy. cFn was also found in rheumatoid synovial fluid by immunoblotting. Approximately one-fifth of the T lymphocytes from rheumatoid synovial fluid bound to Fn. The binding of synovial fluid T cells was always higher than that from peripheral blood. These results have two implications. On the one hand, the cellular type of Fn may be an indicator of synovial inflammation. On the other hand, the deposition of Fn may be a factor contributing to the infiltration of mononuclear cells into the synovium.
针对含有ED序列的细胞纤连蛋白（cFn）的小鼠单克隆抗体被用于证明，炎症滑膜中的纤连蛋白（Fn）与血浆纤连蛋白（pFn）的主要形式不同。通过免疫荧光显微镜检查，在类风湿性滑膜增生部位和滑膜小血管壁中可见cFn的积聚。通过免疫印迹法也在类风湿性滑液中发现了cFn。类风湿性滑液中约五分之一的T淋巴细胞与Fn结合。滑液T细胞的结合总是高于外周血T细胞。这些结果有两个含义。一方面，Fn的细胞类型可能是滑膜炎症的一个指标。另一方面，Fn的沉积可能是导致单核细胞浸润滑膜的一个因素。
