Vartio T, Vaheri A, Von Essen R, Isomäki H, Stenman S
Eur J Clin Invest. 1981 Jun;11(3):207-12. doi: 10.1111/j.1365-2362.1981.tb01842.x.
Fibronectin is a glycoprotein found in body fluids, loose connective tissue matrix and in basement membranes. Fibronectin in rheumatoid arthritis synovial fluid was immunologically indistinguishable from the plasma form, as shown by double-diffusion analysis. Fibronectin isolated from rheumatoid synovial fluid by affinity chromatography on gelatin--Sepharose had a polypeptide pattern similar to that of plasma fibronectin in SDS--polyacrylamide gel electrophoresis. In fifty-one patients with rheumatoid arthritis and related diseases fibronectin concentrations is synovial fluid were 445 +/- 103 micrograms/ml (mean +/- SD) and within normal range, 335 +/- 52 micrograms/ml, in plasma. Immunofluorescence staining showed a prominent increase of fibronectin in the proliferating synovial connective tissue in rheumatoid arthritis as compared to normal synovial membrane. The results suggest an increased local production of fibronectin in rheumatoid synovial tissue.
纤连蛋白是一种存在于体液、疏松结缔组织基质和基底膜中的糖蛋白。如双向扩散分析所示，类风湿性关节炎滑液中的纤连蛋白在免疫学上与血浆形式无法区分。通过在明胶-琼脂糖上进行亲和层析从类风湿性滑液中分离出的纤连蛋白，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的多肽图谱与血浆纤连蛋白相似。在51例类风湿性关节炎及相关疾病患者中，滑液中纤连蛋白浓度为445±103微克/毫升（平均值±标准差），处于血浆正常范围335±52微克/毫升之内。免疫荧光染色显示，与正常滑膜相比，类风湿性关节炎中增殖的滑膜结缔组织中纤连蛋白显著增加。结果表明类风湿性滑膜组织中纤连蛋白的局部产生增加。
