Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Cracow, Poland.
Biol Trace Elem Res. 2013 May;152(2):219-24. doi: 10.1007/s12011-013-9608-9. Epub 2013 Jan 19.
Exposure to substance toxicity is especially dangerous for diabetics because it accelerates and intensifies diabetic complication. Homeostasis of trace elements can be disrupted by diabetes mellitus. On the other hand, disturbance in trace element status in diabetes mellitus may contribute to insulin resistance and development of diabetic complications. The aim of the present study was to compare the concentration of elements in the brain, liver, and kidneys of animals with induced diabetes after the administration of plant preparations (iscador and vincristine) and 5-fluorouracil. The experiments were carried out on male mice. The animals were divided into five groups of ten mice each: one control and four experimental groups. The first experimental group was administered alloxan at 75 mg/kg b.w. for 4 days, the second group was administered both alloxan at 75 mg/kg b.w. and vincristine 1 mg/kg b.w. for 4 days, and the third group was administered both alloxan at 75 mg/kg b.w. and 5-fluorouracil 75 mg/kg b.w. for 4 days. The animals of the fourth group were administered both alloxan at 75 mg/kg b.w. and iscador Qu at 5 mg/kg b.w. for 4 days. Calcium, magnesium, iron, copper, zinc, sodium, and potassium levels in the tissues were analyzed by flame atomic absorption spectrophotometer. We observed that zinc, copper, magnesium, sodium, and potassium were lower in the brain as compared to the control animals. The copper levels in the liver were also lower in diabetic groups than in control groups. However, the iscador and vincristine and 5-fluorouracil did not induce significant differences in the five groups. In conclusion, results of the current study indicated that changes of the investigated essential elements may contribute to explaining the role of impaired element metabolism of some elements in the progression of diabetic complications.
暴露于物质毒性对糖尿病患者尤其危险，因为它会加速和加剧糖尿病并发症。微量元素的动态平衡可能会因糖尿病而受到干扰。另一方面，糖尿病患者微量元素状态的紊乱可能导致胰岛素抵抗和糖尿病并发症的发展。本研究的目的是比较诱导糖尿病后给予植物制剂（iscador 和长春新碱）和 5-氟尿嘧啶的动物的大脑、肝脏和肾脏中的元素浓度。实验在雄性小鼠上进行。将动物分为五组，每组 10 只：一组对照和四组实验组。第一实验组连续 4 天给予 75mg/kg 体重的链脲佐菌素，第二实验组连续 4 天给予 75mg/kg 体重的链脲佐菌素和 1mg/kg 体重的长春新碱，第三实验组连续 4 天给予 75mg/kg 体重的链脲佐菌素和 75mg/kg 体重的 5-氟尿嘧啶。第四实验组连续 4 天给予 75mg/kg 体重的链脲佐菌素和 5mg/kg 体重的 iscador Qu。采用火焰原子吸收分光光度计分析组织中的钙、镁、铁、铜、锌、钠和钾水平。我们观察到与对照组相比，大脑中的锌、铜、镁、钠和钾含量较低。糖尿病组的肝脏铜含量也低于对照组。然而，iscador 和长春新碱和 5-氟尿嘧啶在五组之间没有引起显著差异。总之，本研究结果表明，所研究的必需元素的变化可能有助于解释某些元素代谢受损在糖尿病并发症进展中的作用。
