Orthopedics Department, The Second Affiliated Hospital Zhejiang University College of Medicine, No. 88 Jiefang Road, Hangzhou 310009, People's Republic of China.
Med Oncol. 2013 Mar;30(1):389. doi: 10.1007/s12032-012-0389-3. Epub 2013 Jan 19.
Osteoprotegerin gene (OPG) is an important candidate gene of osteoporosis. The objective of this study was to evaluate the association between OPG gene polymorphisms and bone mineral density (BMD). A total of 336 Chinese postmenopausal women were included in this study. OPG gene polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), created restriction site-PCR (CRS-PCR) and DNA sequencing methods. BMD was evaluated at the lumbar spine (L(2-4)), total hip and femoral neck. Two single-nucleotide polymorphisms (SNPs) (g.21775C>T and g.23367T>C) were identified and the association analysis showed significant difference of spine BMD among different g.23367T>C genotype, subjects with the genotype TT was significantly higher than those of genotype TC and CC. Such a significant difference was not observed at the neck hip BMD and total hip BMD. The g.21775C>T polymorphism was not significantly associated with spine BMD, total hip BMD and neck hip BMD in the studied subjects. These findings suggested that OPG gene polymorphisms were associated with BMD in Chinese postmenopausal women. Results from this study will be helpful in further studies to determine the role of OPG gene in osteoporosis.
骨保护素基因（OPG）是骨质疏松症的一个重要候选基因。本研究旨在评估 OPG 基因多态性与骨密度（BMD）之间的关系。共纳入 336 例中国绝经后妇女。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）、创建限制性位点-PCR（CRS-PCR）和 DNA 测序方法检测 OPG 基因多态性。在腰椎（L2-4）、全髋和股骨颈评估 BMD。鉴定出两个单核苷酸多态性（SNPs）（g.21775C>T 和 g.23367T>C），关联分析显示不同 g.23367T>C 基因型之间脊柱 BMD 存在显著差异，基因型 TT 的受试者明显高于基因型 TC 和 CC 的受试者。在颈髋 BMD 和全髋 BMD 中未观察到这种显著差异。g.21775C>T 多态性与研究对象的脊柱 BMD、全髋 BMD 和颈髋 BMD 无显著相关性。这些发现表明 OPG 基因多态性与中国绝经后妇女的 BMD 相关。本研究结果将有助于进一步研究确定 OPG 基因在骨质疏松症中的作用。
