The relationship between osteoprotegerin gene polymorphisms and bone mineral density in Chinese postmenopausal women.

Previous evidence supports that the osteoprotegerin (OPG) gene is one of the most important candidate genes for influencing the pathogenesis of osteoporosis. The objective of this study was to investigate the relationship between OPG gene polymorphisms and osteoporosis in Chinese postmenopausal women. A total of 764 subjects were included in this study. The bone mineral density (BMD) in the lumbar spine (L2-4), neck hip and total hip was determined by dual-energy X-ray absorptiometry (DEXA). The g.19190C>A and g.25602A>G SNPs were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), created restriction site PCR (CRS-PCR) and DNA sequencing methods. As for g.19190C>A, our data suggested that the BMD value of lumbar spine (L2-4), neck hip and total hip for subjects with CC genotype was significantly higher than that of CA and AA genotypes (P<0.05). No significant difference was detected between the association of g.25602A>G genotypes with spine BMD and neck hip BMD, while total hip BMD almost reached the significant level (P=0.063). These findings provide more evidence that the SNPs in OPG gene could affect BMD and osteoporosis, and the allele-A of g.19190C>A and allele-G of g.25602A>G genetic variants are associated with increased risk for osteoporosis in Chinese postmenopausal women.