Department of Orthopedics, The People's Hospital of Maoming City, Maoming, Guangdong province, People's Republic of China.
Int Immunopharmacol. 2013 Oct;17(2):404-7. doi: 10.1016/j.intimp.2013.06.031. Epub 2013 Jul 13.
Previous evidence supports that the osteoprotegerin (OPG) gene is one of the most important candidate genes for influencing the pathogenesis of osteoporosis. The objective of this study was to investigate the relationship between OPG gene polymorphisms and osteoporosis in Chinese postmenopausal women. A total of 764 subjects were included in this study. The bone mineral density (BMD) in the lumbar spine (L2-4), neck hip and total hip was determined by dual-energy X-ray absorptiometry (DEXA). The g.19190C>A and g.25602A>G SNPs were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), created restriction site PCR (CRS-PCR) and DNA sequencing methods. As for g.19190C>A, our data suggested that the BMD value of lumbar spine (L2-4), neck hip and total hip for subjects with CC genotype was significantly higher than that of CA and AA genotypes (P<0.05). No significant difference was detected between the association of g.25602A>G genotypes with spine BMD and neck hip BMD, while total hip BMD almost reached the significant level (P=0.063). These findings provide more evidence that the SNPs in OPG gene could affect BMD and osteoporosis, and the allele-A of g.19190C>A and allele-G of g.25602A>G genetic variants are associated with increased risk for osteoporosis in Chinese postmenopausal women.
先前的证据表明，护骨素（OPG）基因是影响骨质疏松症发病机制的最重要候选基因之一。本研究旨在探讨 OPG 基因多态性与中国绝经后妇女骨质疏松症的关系。本研究共纳入 764 例受试者。采用双能 X 线吸收法（DEXA）测定腰椎（L2-4）、颈髋和全髋骨密度（BMD）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）、创建限制性位点 PCR（CRS-PCR）和 DNA 测序方法检测 g.19190C>A 和 g.25602A>G SNP。对于 g.19190C>A，我们的数据表明 CC 基因型受试者的腰椎（L2-4）、颈髋和全髋 BMD 值明显高于 CA 和 AA 基因型（P<0.05）。g.25602A>G 基因型与脊柱 BMD 和颈髋 BMD 无显著相关性，而全髋 BMD 几乎达到显著水平（P=0.063）。这些发现提供了更多证据表明，OPG 基因中的 SNP 可能影响 BMD 和骨质疏松症，g.19190C>A 的等位基因-A 和 g.25602A>G 的等位基因-G 与中国绝经后妇女骨质疏松症的风险增加相关。
