Department of Orthopaedic Surgery, No. 98 Hospital of PLA, Huzhou 313000, People's Republic of China.
Endocr J. 2013;60(12):1303-7. doi: 10.1507/endocrj.ej13-0308. Epub 2013 Sep 25.
The objective of this study was to evaluate the association of single nucleotide polymorphisms (SNPs) of osteoprotegerin gene (OPG) with bone mineral density (BMD) and osteoporosis. A total of 338 Chinese postmenopausal women with primary osteoporosis and 367 healthy controls were enrolled. The lumbar spine (L₂₋₄), total hip and femoral neck hip of BMD were assessed by dual-energy X-ray absorptiometry (DEXA). OPG genetic variants were genotyped through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), created restriction site-PCR (CRS-PCR) and DNA sequencing methods. In this study, the g.18861A>G and g.25548C>T SNPs were detected and our data suggested that the significant differences of spine BMD, femoral neck hip BMD and total hip BMD were found among different g.18861A>G genotype, subjects with the AA genotype were significantly higher than those of AG and GG genotypes (p < 0.05). The g.25548C>T variant was not significantly associated with spine BMD, femoral neck hip BMD and total hip BMD (p > 0.05), while almost reached at the significant level in total hip BMD (p = 0.061). These findings suggeste that OPG gene variants are related to BMD and osteoporosis in Chinese postmenopausal women.
本研究旨在评估骨保护素基因（OPG）单核苷酸多态性（SNPs）与骨密度（BMD）和骨质疏松症的相关性。共纳入 338 例原发性骨质疏松症的绝经后中国女性和 367 名健康对照者。采用双能 X 线吸收法（DEXA）评估腰椎（L₂₋₄）、全髋和股骨颈骨密度。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）、创建限制性位点-PCR（CRS-PCR）和 DNA 测序方法对 OPG 遗传变异进行基因分型。在本研究中，检测到 g.18861A>G 和 g.25548C>T SNP，我们的数据表明，不同 g.18861A>G 基因型之间的脊柱 BMD、股骨颈 BMD 和全髋 BMD 存在显著差异，AA 基因型的受试者明显高于 AG 和 GG 基因型（p < 0.05）。g.25548C>T 变体与脊柱 BMD、股骨颈 BMD 和全髋 BMD 无显著相关性（p > 0.05），而在全髋 BMD 中几乎达到显著水平（p = 0.061）。这些发现提示 OPG 基因变异与中国绝经后妇女的 BMD 和骨质疏松症有关。
