Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
Biotechnol Bioeng. 2013 Aug;110(8):2096-104. doi: 10.1002/bit.24844. Epub 2013 Feb 9.
The lipid characteristics of microalgae are known to differ between species and change with growth conditions. This work provides a methodology for lipid characterization that enables selection of the optimal strain, cultivation conditions, and processing pathway for commercial biodiesel production from microalgae. Two different microalgal species, Nannochloropsis sp. and Chlorella sp., were cultivated under both nitrogen replete and nitrogen depleted conditions. Lipids were extracted and fractionated into three major classes and quantified gravimetrically. The fatty acid profile of each fraction was analyzed using GC-MS. The resulting quantitative lipid data for each of the cultures is discussed in the context of biodiesel and omega-3 production. This approach illustrates how the growth conditions greatly affect the distribution of fatty acid present in the major lipid classes and therefore the suitability of the lipid extracts for biodiesel and other secondary products.
微藻的脂质特性在不同物种之间存在差异，并随生长条件而变化。本工作提供了一种脂质特征分析的方法，可用于选择最佳的微藻菌株、培养条件和加工途径，以用于商业生物柴油生产。两种不同的微藻物种，即 Nannochloropsis sp. 和 Chlorella sp.，在氮充足和氮缺乏的条件下进行培养。提取脂质并分成三个主要类别，并通过重量法进行定量。使用 GC-MS 分析每个馏分的脂肪酸组成。讨论了每种培养物的定量脂质数据，以生物柴油和 omega-3 生产为背景。这种方法说明了生长条件如何极大地影响主要脂质类别中存在的脂肪酸分布，从而影响脂质提取物用于生物柴油和其他次级产品的适用性。
