核糖体 RNA (rrn) 操纵子工程枯草芽孢杆菌作为一种可行的抗生素发现的测试生物。
rRNA (rrn) operon-engineered Bacillus subtilis as a feasible test organism for antibiotic discovery.
机构信息
Department of Life Science, Hiroshima Institute of Technology, Saeki-ku, Hiroshima, Japan.
出版信息
Antimicrob Agents Chemother. 2013 Apr;57(4):1948-51. doi: 10.1128/AAC.02604-12. Epub 2013 Jan 18.
Abstract
Bacillus subtilis contains 10 rRNA (rrn) operons. We found that rRNA operon-engineered B. subtilis strain RIK543, with only the rrnO operon, is specifically hypersensitive to RNA polymerase inhibitors such as rifamycin SV and rifampin (80-fold and 20-fold, respectively). In pilot screening experiments, we found actinomycete isolates successfully at an incidence of 1.9% (18/945) that produced antibacterials that were detectable only with RIK543 as the test organism. Strain RIK543 may be a feasible test organism for the discovery of novel RNA polymerase inhibitors.
摘要
枯草芽孢杆菌含有 10 个 rRNA（rrn）操纵子。我们发现，仅 rrnO 操纵子工程化的枯草芽孢杆菌 RIK543 菌株对 RNA 聚合酶抑制剂如利福霉素 SV 和利福平（分别为 80 倍和 20 倍）特别敏感。在初步筛选实验中，我们发现放线菌分离物的成功发生率为 1.9%（18/945），这些分离物产生的抗生素只能用 RIK543 作为测试生物来检测。RIK543 菌株可能是发现新型 RNA 聚合酶抑制剂的可行测试生物。
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