Craney Arryn, Ozimok Cory, Pimentel-Elardo Sheila Marie, Capretta Alfredo, Nodwell Justin R
Michael Degroote Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, Ontario L8N 3Z5, Canada.
Chem Biol. 2012 Aug 24;19(8):1020-7. doi: 10.1016/j.chembiol.2012.06.013.
Bacterially produced secondary metabolites are used as antibiotics, anticancer drugs, and for many other medicinal applications. The mechanisms that limit the production of these molecules in the laboratory are not well understood, and this has impeded the discovery of many important compounds. We have identified small molecules that remodel the yields of secondary metabolites in many actinomycetes and show that one set of these molecules does so by inhibiting fatty acid biosynthesis. This demonstrates a particularly intimate relationship between this primary metabolic pathway and secondary metabolism and suggests an approach to enhance the yields of metabolites for discovery and biochemical characterization.
细菌产生的次生代谢产物被用作抗生素、抗癌药物以及许多其他医学用途。在实验室中限制这些分子产生的机制尚未得到充分理解，这阻碍了许多重要化合物的发现。我们已经鉴定出能重塑许多放线菌中次生代谢产物产量的小分子，并表明其中一组小分子是通过抑制脂肪酸生物合成来实现这一作用的。这证明了这条初级代谢途径与次生代谢之间存在特别密切的关系，并提出了一种提高代谢产物产量以用于发现和生化表征的方法。
