苔藓（紫萼藓和小立碗藓）重组非共生血红蛋白的光谱分析。
Spectroscopic analysis of moss (Ceratodon purpureus and Physcomitrella patens) recombinant non-symbiotic hemoglobins.
作者信息
Vázquez-Limón Consuelo, Castro-Bustos Saraí, Arredondo-Peter Raúl
机构信息
Laboratorio de Biofísica y Biología Molecular; Departamento de Bioquímica y Biología Molecular; Facultad de Ciencias; Universidad Autónoma del Estado de Morelos; Cuernavaca, Morelos México.
出版信息
Commun Integr Biol. 2012 Nov 1;5(6):527-30. doi: 10.4161/cib.21473.
Non-symbiotic hemoglobins (nsHbs) are O(2)-binding proteins widely distributed in land plants, including primitive bryophytes. Little is known about the properties of bryophyte nsHbs. Here, we report the spectroscopic characterization of two moss recombinant nsHbs, CerpurnsHb of Ceratodon purpureus and PhypatnsHb of Physcomitrella patens. Spectra showed that the absorption maxima of the ferrous and ferric forms of recombinant CerpurnsHb are located at 418, 531 and 557 nm and 407, 537, 569 (shoulder) and 632 (shoulder) nm, respectively, and of PhypatnsHb are located at 422, 529 and 557 nm and 407, 531, 571 (shoulder) and 647 (shoulder) nm, respectively. These absorption maxima are similar to those of rice Hb1. Also, the absorption maxima of the oxygenated ferrous form of recombinant CerpurnsHb and PhypatnsHb are located at 412, 541 and 575 nm and 414, 541 and 574 nm, respectively, similar to those of oxygenated rice Hb1 and cowpea leghemoglobin II. This evidence indicates that CerpurnsHb and PhypatnsHb are mostly hexacoordinate and that they bind O(2).
非共生血红蛋白（nsHbs）是广泛分布于陆地植物（包括原始苔藓植物）中的O₂结合蛋白。关于苔藓植物nsHbs的特性知之甚少。在此，我们报道了两种苔藓重组nsHbs的光谱特征，即紫萼藓（Ceratodon purpureus）的CerpurnsHb和小立碗藓（Physcomitrella patens）的PhypatnsHb。光谱显示，重组CerpurnsHb的亚铁和高铁形式的吸收峰分别位于418、531和557 nm以及407、537、569（肩峰）和632（肩峰）nm，而PhypatnsHb的吸收峰分别位于422、529和557 nm以及407、531、571（肩峰）和647（肩峰）nm。这些吸收峰与水稻Hb1的吸收峰相似。此外，重组CerpurnsHb和PhypatnsHb的氧合亚铁形式的吸收峰分别位于412、541和575 nm以及414、541和574 nm，与氧合水稻Hb1和豇豆豆血红蛋白II的吸收峰相似。这一证据表明CerpurnsHb和PhypatnsHb大多为六配位且它们能结合O₂。
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