拟南芥中的两个血红蛋白基因：豆血红蛋白的进化起源。
Two hemoglobin genes in Arabidopsis thaliana: the evolutionary origins of leghemoglobins.
作者信息
Trevaskis B, Watts R A, Andersson C R, Llewellyn D J, Hargrove M S, Olson J S, Dennis E S, Peacock W J
机构信息
Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia.
出版信息
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):12230-4. doi: 10.1073/pnas.94.22.12230.
We cloned two hemoglobin genes from Arabidopsis thaliana. One gene, AHB1, is related in sequence to the family of nonsymbiotic hemoglobin genes previously identified in a number of plant species (class 1). The second hemoglobin gene, AHB2, represents a class of nonsymbiotic hemoglobin (class 2) related in sequence to the symbiotic hemoglobin genes of legumes and Casuarina. The properties of these two hemoglobins suggest that the two families of nonsymbiotic hemoglobins may differ in function from each other and from the symbiotic hemoglobins. AHB1 is induced, in both roots and rosette leaves, by low oxygen levels. Recombinant AHB1 has an oxygen affinity so high as to make it unlikely to function as an oxygen transporter. AHB2 is expressed at a low level in rosette leaves and is low temperature-inducible. AHB2 protein has a lower affinity for oxygen than AHB1 but is similar to AHB1 in having an unusually low, pH-sensitive oxygen off-rate.
我们从拟南芥中克隆了两个血红蛋白基因。其中一个基因AHB1，其序列与先前在许多植物物种中鉴定出的非共生血红蛋白基因家族（第1类）相关。第二个血红蛋白基因AHB2，代表一类非共生血红蛋白（第2类），其序列与豆科植物和木麻黄的共生血红蛋白基因相关。这两种血红蛋白的特性表明，这两个非共生血红蛋白家族在功能上可能彼此不同，且与共生血红蛋白也不同。AHB1在根和莲座叶中均受低氧水平诱导。重组AHB1具有极高的氧亲和力，以至于不太可能作为氧转运蛋白发挥作用。AHB2在莲座叶中低水平表达，且受低温诱导。AHB2蛋白对氧的亲和力低于AHB1，但在具有异常低的、对pH敏感的氧解离速率方面与AHB1相似。
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