Department of Urology, Medical University of Vienna, Vienna, Austria.
Urology. 2013 Apr;81(4):869-72. doi: 10.1016/j.urology.2012.12.011. Epub 2013 Jan 18.
To evaluate the prevalence and genotypes of high-risk human papilloma virus (HPV) infection of the foreskin in asymptomatic boys before first sexual intercourse.
We collected 50 consecutive foreskin specimens after radical circumcision. Indication for surgery was phimosis. High-risk HPV status was determined by real-time polymerase chain reaction for the genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59. Immunohistochemistry for p16(INK4a) was performed.
The median age at the time of surgery was 5.5 years (range, 5 months-15 years). High-risk HPV was detected in 6 of 50 foreskins (12%). All positive samples showed HPV16. No association with age or grade of phimosis was observed. Two samples were focally positive for p16(INK4a), both of which were HPV-negative.
In a significant proportion of boys, subclinical high-risk HPV infections are found in the foreskin, which could be a reservoir for HPV-associated diseases. Our study generates the hypothesis that nonsexual routes play significant roles in HPV transmission. Because the human foreskin represents a high-risk HPV reservoir, vaccination may be also advised in boys.
评估首次性行为前无症状男童包皮中高危型人乳头瘤病毒（HPV）感染的流行率和基因型。
我们收集了 50 例连续的包皮环切术后标本。手术指征为包茎。通过实时聚合酶链反应检测 HPV16、18、31、33、35、39、45、51、52、56、58 和 59 型高危 HPV 状态。进行 p16（INK4a）免疫组化染色。
手术时的中位年龄为 5.5 岁（范围，5 个月至 15 岁）。50 个包皮中有 6 个（12%）检测到高危 HPV。所有阳性样本均显示 HPV16。未观察到 HPV 感染与年龄或包茎程度之间的相关性。2 个样本局灶性 p16（INK4a）阳性，均为 HPV 阴性。
在相当一部分男孩中，包皮中存在亚临床高危型 HPV 感染，这可能是 HPV 相关疾病的潜在传染源。本研究提出了一个假设，即非性传播途径在 HPV 传播中起重要作用。由于人类包皮是高危 HPV 的储存库，因此也可以建议男孩接种疫苗。
