Department of Urology, Harran University School of Medicine, Sanliurfa, Turkey.
Urology. 2012 Aug;80(2):423-6. doi: 10.1016/j.urology.2012.02.049. Epub 2012 May 2.
To evaluate whether the high-risk human papillomavirus (HPV) subtypes that are strongly related to cervical cancer are harbored in the prepuce of the circumcised tissue of prepubertal boys in the period just before active sexual life.
The present study enrolled 30 healthy boys (age range 4-11 years, mean age 8.1 ± 1.6) who underwent a standard circumcision procedure, with hypospadias repair in 3 patients. All prepuceal samples were studied using real-time polymerase chain reaction and grouped according to HPV subtype prevalence as groups 1 (types 16 and 18), 2 (types 31, 33, 45, 52, and 58), and 3 (types 35, 39, 51, 56, 59, 66, and 68).
HPV DNA was reported in 25 (83.3%) of the 30 subjects. All samples showed a negative result for group 2. Although most of the positive findings were for group 3 (25 [83.3%] of 30), a positive result was reported for only 1 subject for group 1 (3.3%).
The results of the present study have shown that the prepuce harbored the rarest HPV types, including types 35, 39, 51, 56, 59, 66, and 68 in preadolescence boys with a high rate (83%). These findings are in contrast to the common knowledge of HPV prevalence in adults that points to the dominance of HPV subtypes 16 and 18.
评估高危型人乳头瘤病毒（HPV）与宫颈癌密切相关的亚型是否存在于青春期前男孩行包皮环切术前的包皮组织中。
本研究纳入了 30 名健康男孩（年龄 4-11 岁，平均年龄 8.1±1.6 岁），他们接受了标准的包皮环切术，其中 3 例伴有尿道下裂修复。所有包皮样本均采用实时聚合酶链反应进行研究，并根据 HPV 亚型流行率分为 1 组（16 型和 18 型）、2 组（31、33、45、52 和 58 型）和 3 组（35、39、51、56、59、66 和 68 型）。
30 名受试者中有 25 名（83.3%）报告 HPV DNA 阳性。所有样本组 2 均为阴性。尽管大多数阳性发现为组 3（30 例中的 25 例，83.3%），但组 1 仅报告了 1 例阳性（3.3%）。
本研究结果表明，在青春期前男孩的包皮中存在包括 35、39、51、56、59、66 和 68 型在内的最罕见 HPV 类型，其阳性率很高（83%）。这些发现与成人 HPV 流行率的普遍认识相反，成人 HPV 流行率以 HPV 亚型 16 和 18 为主导。
