Promega Corporation, Madison, WI 53711, United States.
Forensic Sci Int Genet. 2013 Feb;7(2):240-50. doi: 10.1016/j.fsigen.2012.10.013. Epub 2013 Jan 20.
The PowerPlex® Y23 System combines the seventeen Y-STR loci in current commercially available Y-STR kits (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4) with six new highly discriminating Y-STR loci (DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643). These six new loci have higher gene diversities than most of the loci in other commercial Y-STR analysis kits, allowing for further distinction between unrelated male individuals. In addition, the inclusion of two rapidly mutating Y-STR loci may allow for the discrimination of related individuals. The PowerPlex® Y23 System is designed to amplify DNA from purified extracts as well as direct amplification from substrates used to collect database samples (e.g. swabs and storage cards). Validation of the PowerPlex® Y23 System includes all of the studies required by the FBI and SWGDAM. The results demonstrate that the PowerPlex® Y23 System is a robust and reliable amplification kit capable of overcoming high concentrations of commonly encountered inhibitors such as hematin, humic acid, and tannic acid. Full profiles are consistently detected with 62.5 pg of male DNA, even in the presence of excessive amounts of female DNA, establishing the PowerPlex(®) Y23 System as a sensitive method for Y-STR testing. Complete Y-STR profiles are detected from mixed samples with 62.5 pg of male DNA in a background of 400 ng of female DNA or 125 pg of male DNA mixed with 3000 ng of female DNA.
PowerPlex® Y23 系统结合了目前市场上可购买的 Y-STR 试剂盒中的十七个 Y-STR 基因座（DYS19、DYS385a/b、DYS389I/II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS635 和 Y-GATA-H4）与六个新的高分辨 Y-STR 基因座（DYS481、DYS533、DYS549、DYS570、DYS576 和 DYS643）。这六个新的基因座具有比大多数其他商业 Y-STR 分析试剂盒中的基因座更高的基因多样性，使得在无关男性个体之间能够进一步区分。此外，包含两个快速突变的 Y-STR 基因座可能允许区分相关个体。PowerPlex® Y23 系统旨在扩增来自纯化提取物以及用于收集数据库样本的基质（例如拭子和存储卡）的 DNA。PowerPlex® Y23 系统的验证包括 FBI 和 SWGDAM 要求的所有研究。结果表明，PowerPlex® Y23 系统是一种强大且可靠的扩增试剂盒，能够克服常见抑制剂（如血红素、腐殖酸和单宁酸）的高浓度。即使存在大量女性 DNA，也能始终如一地检测到 62.5pg 的男性 DNA，从而建立了 PowerPlex(®) Y23 系统作为一种用于 Y-STR 测试的敏感方法。即使在背景中有 400ng 女性 DNA 或 125pg 男性 DNA 与 3000ng 女性 DNA 混合的情况下，也能从混合样本中检测到完整的 Y-STR 图谱，混合样本中含有 62.5pg 的男性 DNA。
