Promega Corporation, Madison, WI, USA.
Methods Mol Biol. 2023;2685:227-239. doi: 10.1007/978-1-0716-3295-6_14.
The PowerPlex Y23 System offered by Promega Corporation contains 23 Y-STR loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and Y-GATA-H4). The PowerPlex Y23 System is designed to amplify DNA from purified extracts as well as direct amplification from substrates used to collect database samples (e.g., swabs and storage cards). Protocols are provided for full-volume reactions for DNA extracts, as well as half-volume reactions for direct amplifications from different substrates.
Promega 公司的 PowerPlex Y23 系统包含 23 个 Y-STR 基因座(DYS19、DYS385a/b、DYS389I/II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS481、DYS533、DYS549、DYS570、DYS576、DYS635、DYS643 和 Y-GATA-H4)。PowerPlex Y23 系统旨在扩增来自纯化提取物的 DNA,以及直接从用于收集数据库样本的基质(例如拭子和存储卡)进行扩增。该系统提供了完整体积反应的 DNA 提取协议,以及来自不同基质的半体积直接扩增协议。