Department of Chemistry, University of New Hampshire, Durham, 03824, USA.
Sensors (Basel). 2013 Jan 21;13(1):1341-52. doi: 10.3390/s130101341.
Microparticles consisting of the thermal responsive polymer N-isopropyl acrylamide (polyNIPAM), a metal ion-binding ligand and a fluorophore pair that undergoes fluorescence resonance energy transfer (FRET) have been prepared and characterized. Upon the addition of Cu(II), the microparticles swell or contract depending on whether charge is introduced or neutralized on the polymer backbone. The variation in microparticle morphology is translated into changes in emission of each fluorophore in the FRET pair. By measuring the emission intensity ratio between the FRET pair upon Cu(II) addition, the concentration of metal ion in solution can be quantified. This ratiometric fluorescent indicator is the newest technique in an ongoing effort to use emission spectroscopy to monitor Cu(II) thermodynamic activity in environmental water samples.
已制备并表征了由热响应聚合物 N-异丙基丙烯酰胺（polyNIPAM）、金属离子结合配体和荧光团对组成的微粒，该荧光团对发生荧光共振能量转移（FRET）。加入 Cu(II)后，聚合物主链上引入或中和电荷，微粒会发生膨胀或收缩。微粒形态的变化转化为 FRET 对中每个荧光团的发射变化。通过测量加入 Cu(II)后 FRET 对的发射强度比，可以定量溶液中金属离子的浓度。这种比率荧光指示剂是使用发射光谱法监测环境水样中 Cu(II)热力学活性的最新技术。
