Department of Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1a, A-6020 Innsbruck, Austria.
Neuroscience. 2013 Apr 16;236:345-72. doi: 10.1016/j.neuroscience.2013.01.008. Epub 2013 Jan 18.
The GABAA receptor is the main inhibitory receptor in the brain and its subunits originate from different genes or gene families (α1-α6, β1-β3, γ1-γ3, δ, ε, θ, π, or ρ1-3). In the mouse brain the anatomical distribution of GABAA receptor subunit mRNAs so far investigated is restricted to subunits forming benzodiazepine-sensitive receptor complexes (α1-α3, α5, β2, β3 and γ2) in the forebrain and midbrain as assessed by in situ hybridization (ISH). In the present study the anatomical distribution of the GABAA receptor subunits α1-α6, β1-β3, γ1-γ2 and δ was analyzed in the mouse brain (excluding brain stem) by ISH and immunohistochemistry (IHC). In several brain areas such as hippocampus, cerebellum, bulbus olfactorius and habenula we observed that mRNA levels did not reflect protein levels, indicating that the protein is located far distantly from the cell body. We also compared the distribution of these 12 subunit mRNAs and proteins with that reported in the rat brain. Although in general there is a considerable correspondence in the distribution between mouse and rat brains, several species-specific differences were observed.
GABAA 受体是大脑中的主要抑制性受体，其亚基源自不同的基因或基因家族（α1-α6、β1-β3、γ1-γ3、δ、ε、θ、π或 ρ1-3）。在小鼠大脑中，迄今为止通过原位杂交（ISH）研究的 GABAA 受体亚基 mRNA 的解剖分布仅限于前脑和中脑中形成苯二氮䓬敏感受体复合物的亚基（α1-α3、α5、β2、β3 和 γ2）。在本研究中，通过 ISH 和免疫组织化学（IHC）分析了小鼠大脑（不包括脑干）中 GABAA 受体亚基 α1-α6、β1-β3、γ1-γ2 和 δ 的解剖分布。在海马体、小脑、嗅球和缰核等几个脑区，我们观察到 mRNA 水平并未反映蛋白水平，表明蛋白位于远离细胞体的地方。我们还比较了这些 12 个亚基 mRNA 和蛋白的分布与大鼠脑中的分布。尽管在小鼠和大鼠大脑之间的分布通常存在相当大的一致性，但也观察到了一些种特异性差异。
