Division of Clinical Biochemistry, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, Canada.
Clin Biochem. 2013 May;46(7-8):642-51. doi: 10.1016/j.clinbiochem.2013.01.002. Epub 2013 Jan 18.
To develop an accurate assay and establish the normal reference intervals for serum cortisol, corticosterone, 11-deoxycortisol, androstenedione, 21-hydroxyprogesterone, testosterone, 17-hydroxyprogesterone, and progesterone. These steroids are commonly used as biomarkers for the diagnosis and monitoring of endocrine diseases such as congenital adrenal hyperplasia. Appropriate age- and gender-stratified reference intervals are essential in accurate interpretation of steroid hormone levels.
The samples analyzed in this study were collected from healthy, ethnically diverse children in the Greater Toronto Area as part of the CALIPER program. A total of 337 serum samples from children between the ages of 0 and 18years were analyzed. The concentrations were measured by using an LC-MS/MS method. The data were analyzed for outliers and age- and gender-specific partitions were established prior to establishing the 2.5th and 97.5th percentiles for the reference intervals.
Reference intervals for all hormones required significant age-dependent stratification while testosterone and progesterone required additional sex-dependent stratification.
We report a sensitive, accurate and relatively fast LC-MS/MS method for the simultaneous measurement of eight steroid hormones. Detailed reference intervals partitioned based on both age and gender were also established for all eight steroid hormones.
开发一种准确的检测方法，并建立血清皮质醇、皮质酮、11-脱氧皮质醇、雄烯二酮、21-羟孕酮、睾酮、17-羟孕酮和孕酮的正常参考区间。这些类固醇通常被用作诊断和监测先天性肾上腺增生等内分泌疾病的生物标志物。在准确解释类固醇激素水平时，适当的按年龄和性别分层的参考区间是必不可少的。
本研究分析的样本来自大多伦多地区的健康、种族多样化的儿童，作为 CALIPER 计划的一部分。共分析了 337 名 0 至 18 岁儿童的血清样本。采用 LC-MS/MS 方法测量浓度。对数据进行离群值分析，并在建立参考区间的 2.5%和 97.5%百分位值之前，按年龄和性别进行特定分区。
所有激素的参考区间都需要进行显著的年龄依赖性分层，而睾酮和孕酮还需要进行额外的性别依赖性分层。
我们报告了一种灵敏、准确且相对快速的 LC-MS/MS 方法，可同时测量八种类固醇激素。还为所有八种类固醇激素建立了基于年龄和性别的详细参考区间分区。
