Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, China.
Biosci Trends. 2012 Dec;6(6):313-24.
To explore the effect of mild hypothermia (35ºC) on breast cancer cells adhesion to vascular endothelial cells, a parallel plant flow chamber was used to observe the adhesion of human breast cancer cells MDA-MB-231 to endothelial cells Eahy926 under physiological flow at 35ºC and 37ºC, as well as the role of intercellular adhesion molecule 1 (ICAM-1) in this process. Further, the effect of mild hypothermia (35ºC) on migration of MDA-MB-231 was also studied. Our results show that mild hypothermia can inhibit the adhesion of tumor cells to endothelial cells and ICAM-1 plays an important role in this process. However, mild hypothermia inhibits breast cancer cell adhesion in a way independent on the change of ICAM-1 expression under our experimental conditions. Mild hypothermia can weaken the chemotaxis of breast cancer cells while it has no obvious effect on unidirectonal migration capacity. These results suggest that mild hypothermia could be used as a potentially adjunct treatment combined with surgery to decrease tumor cell adhesion and migration.
为了探讨轻度低温（35℃）对乳腺癌细胞黏附血管内皮细胞的影响，采用平行流室观察人乳腺癌细胞 MDA-MB-231 在生理流动条件下于 35℃和 37℃时黏附于内皮细胞 Eahy926 的情况，并研究细胞间黏附分子 1（ICAM-1）在此过程中的作用。进一步研究了轻度低温（35℃）对 MDA-MB-231 迁移的影响。我们的结果表明，轻度低温可抑制肿瘤细胞与内皮细胞的黏附，而 ICAM-1 在该过程中起重要作用。然而，在我们的实验条件下，轻度低温抑制乳腺癌细胞黏附的方式与 ICAM-1 表达的变化无关。轻度低温可削弱乳腺癌细胞的趋化性，但对单向迁移能力无明显影响。这些结果提示，轻度低温可作为一种潜在的辅助治疗方法与手术结合，以减少肿瘤细胞黏附和迁移。
