Division of Immunotechnology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
Analyst. 2013 Mar 7;138(5):1427-33. doi: 10.1039/c2an36682g.
A direct immunoassay format with human growth hormone (hGH) immobilized on a self assembled monolayer modified surface plasmon resonance (SPR) chip was chosen to detect specific antibodies (anti-hGH) using different electrochemical techniques. Atomic force microscopy imaging and SPR were used as control methods for the evaluation and confirmation of the antigen-antibody complex formation. The applicability and sensitivity of candidate electrochemical techniques to develop an accurate and sensitive electrochemical immunosensor were investigated. Four electrochemical methods for anti-hGH determination - pulse amperometry (PA), cyclic voltammetry (CV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV), were compared. Higher sensitivity of the developed immunosensor was observed using PA and CV: analytical signal registered using the PA method was 2.50 times higher in comparison with CV, 16.3 times higher in comparison with SWV and 24.5 times higher in comparison with the DPV method. In the case of PA detection method, the limit of detection was lower (75 nM) than that of the CV method (108 nM).
选择了一种将人生长激素（hGH）固定在自组装单层修饰的表面等离子体共振（SPR）芯片上的直接免疫测定方法，使用不同的电化学技术检测特定的抗体（抗 hGH）。原子力显微镜成像和 SPR 被用作评估和确认抗原-抗体复合物形成的对照方法。研究了候选电化学技术在开发准确和灵敏的电化学免疫传感器中的适用性和灵敏度。比较了用于抗 hGH 测定的四种电化学方法-脉冲安培法（PA）、循环伏安法（CV）、方波伏安法（SWV）和差分脉冲伏安法（DPV）。使用 PA 和 CV 观察到开发的免疫传感器具有更高的灵敏度：与 CV 相比，使用 PA 方法记录的分析信号高 2.50 倍，与 SWV 相比高 16.3 倍，与 DPV 方法相比高 24.5 倍。在 PA 检测方法的情况下，检测限较低（75 nM），低于 CV 方法（108 nM）。
