Plant Research International, Wageningen, The Netherlands.
Analyst. 2013 Mar 21;138(6):1619-27. doi: 10.1039/c2an36787d. Epub 2013 Jan 22.
Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains - the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.
检测元件在生物传感器中的分析物识别中起着关键作用。因此，需要具有高分析物特异性和结合强度的检测元件。虽然抗体 (Abs) 已越来越多地被用作生物传感器中的检测元件，但仍然存在一个关键挑战——在生物传感器表面上的固定化。这篇小型综述强调了最近用于固定和研究表面上 Abs 的方法。我们首先介绍了用作检测元件的 Ab 种类，并讨论了最近用于通过确定层厚度或表面拓扑来阐明 Ab 取向的技术。然后，将介绍几种固定化方法：非共价和共价表面附着，产生定向或随机偶联的 Abs。最后，综述了适用于定向 Ab 固定化的蛋白质修饰方法，以期未来的应用。
