Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Centros, Singapore 138668, Singapore.
Appl Microbiol Biotechnol. 2013 Mar;97(5):1865-73. doi: 10.1007/s00253-013-4702-7. Epub 2013 Jan 22.
Pichia yeasts have been recognized as important microbial cell factories in the biotechnological industry. Notably, the Pichia pastoris and Pichia stipitis species have attracted much research interest due to their unique cellular physiology and metabolic capability: P. pastoris has the ability to utilize methanol for cell growth and recombinant protein production, while P. stipitis is capable of assimilating xylose to produce ethanol under oxygen-limited conditions. To harness these characteristics for biotechnological applications, it is highly required to characterize their metabolic behavior. Recently, following the genome sequencing of these two Pichia species, genome-scale metabolic networks have been reconstructed to model the yeasts' metabolism from a systems perspective. To date, there are three genome-scale models available for each of P. pastoris and P. stipitis. In this mini-review, we provide an overview of the models, discuss certain limitations of previous studies, and propose potential future works that can be conducted to better understand and engineer Pichia yeasts for industrial applications.
毕赤酵母已被公认为生物技术产业中重要的微生物细胞工厂。值得注意的是，由于其独特的细胞生理学和代谢能力，巴斯德毕赤酵母和产朊假丝酵母已引起了广泛的研究兴趣：毕赤酵母能够利用甲醇进行细胞生长和重组蛋白生产，而产朊假丝酵母能够在限氧条件下利用木糖生产乙醇。为了利用这些特性进行生物技术应用，非常有必要对其代谢行为进行表征。最近，在这两个毕赤酵母种完成基因组测序后，基于它们的基因组规模代谢网络已经被重建，以便从系统角度对酵母的代谢进行建模。迄今为止，每种毕赤酵母都有三个基因组规模模型。在这篇综述中，我们提供了对这些模型的概述，讨论了以前研究的某些局限性，并提出了可以进行的潜在未来工作，以更好地理解和工程化毕赤酵母，以应用于工业生产。
