Gucu Arif, Cavusoglu Ilkin, Bozkurt Onder, Eris Cuneyt, Toktas Faruk, Goncu Tugrul, Ozyazicioglu Ahmet
Department of Cardiovascular Surgery, Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey.
J Cardiothorac Surg. 2013 Jan 22;8:16. doi: 10.1186/1749-8090-8-16.
Coronary occlusion techniques during OPCAB may lead to an endothelial damage to the target vessel. The adverse effects of these techniques are well-known, and researches have been trying to find out new materials to occlude the coronary artery without an endothelial damage. In the present study, we investigate to the endothelial damage in the rat aorta which is occluded by Poloxamer 407 gel.
Forty-five rats were randomized in three groups: (1) segment of the aorta was occluded with Poloxamer 407 gel in P 407 group; (2) segment of the aorta was occluded with microvascular clamp in MV clamp group; and (3) no onclusion was available in the Control group. The rats were sacrificed of observation, and a 15mm segment of the aorta was obtained as a specimen. Integrity of the endothelial lining was observed with a scanning electron microscopy.
Scanning electron microscopy revealed a statistically significant difference among the 3 groups (p<0,001) using the SPSS 13.0 test. No difference was found between the Control group and the P 407 group (p=0,059). The differences between MV clamp-Control group (p<0,001) and MV clamp-P 407 group were statistically significant (p<0,002).
We suggest that Poloxamer 407 gel occlusion may be a safer and more effective method compared to the microvascular clamp occlusion.
非体外循环冠状动脉搭桥术（OPCAB）期间的冠状动脉闭塞技术可能会导致靶血管内皮损伤。这些技术的不良影响是众所周知的，并且研究一直在试图寻找新的材料来闭塞冠状动脉而不造成内皮损伤。在本研究中，我们调查了用泊洛沙姆407凝胶闭塞的大鼠主动脉中的内皮损伤情况。
45只大鼠被随机分为三组：（1）在P 407组中用泊洛沙姆407凝胶闭塞主动脉段；（2）在微血管夹组中用微血管夹闭塞主动脉段；（3）对照组不进行闭塞操作。处死大鼠进行观察，获取15mm长的主动脉段作为标本。用扫描电子显微镜观察内皮衬里的完整性。
使用SPSS 13.0软件进行检验，扫描电子显微镜显示三组之间存在统计学显著差异（p<0.001）。对照组和P 407组之间未发现差异（p=0.059）。微血管夹-对照组（p<0.001）和微血管夹-P 407组之间的差异具有统计学意义（p<0.002）。
我们认为，与微血管夹闭塞相比，泊洛沙姆407凝胶闭塞可能是一种更安全、更有效的方法。
