Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Vellore District, Tamil Nadu, India.
Asian Pac J Trop Med. 2013 Feb;6(2):95-101. doi: 10.1016/S1995-7645(13)60002-4.
To investigate the larvicidal activity of synthesized silver nanoparticles (Ag NPs) utilizing aqueous bark extract of Ficus racemosa (F. racemosa) was tested against fourth instar larvae of filariasis vector, Culex quinquefasciatus (Cx. quinquefasciatus) and japanese encephalitis vectors, Culex gelidus (Cx. gelidus).
The synthesized Ag NPs was characterized by UV-vis spectrum, X-ray diffraction (XRD), Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR). The larvicidal activities were assessed for 24 h against the larvae of Cx. quinquefasciatus and Cx. gelidus with varying concentrations of aqueous bark extract of F. racemosa and synthesized Ag NPs. LC(50) and r(2) values were calculated.
The maximum efficacy was observed in crude aqueous extract of F. racemosa against the larvae of Cx. quinquefasciatus and Cx. gelidus (LC(50)=67.72 and 63.70 mg/L; r(2)=0.995 and 0.985) and the synthesized Ag NPs (LC(50)=12.00 and 11.21 mg/L; r(2)=0.997 and 0.990), respectively. Synthesized Ag NPs showed the XRD peaks at 2 θ values of 27.61, 29.60, 35.48, 43.48 and 79.68 were identified as (210), (121), (220), (200) and (311) reflections, respectively. The FTIR spectra of Ag NPs exhibited prominent peaks at 3,425, 2,878, 1,627 and 1,382 in the region 500-3,000 cm(-1). The peaks correspond to the presence of a stretching vibration of (NH) C=O group. SEM analysis showed shape in cylindrical, uniform and rod with the average size of 250.60 nm.
The biosynthesis of silver nanoparticles using bark aqueous extract of F. racemosa and its larvicidal activity against the larvae of disease spreading vectors. The maximum larvicidal efficacy was observed in the synthesized Ag NPs.
利用榕属植物水提物合成银纳米粒子（Ag NPs），检测其对致丝虫病媒介埃及伊蚊（Cx. quinquefasciatus）和日本脑炎媒介寒蝉（Cx. gelidus）四龄幼虫的杀幼虫活性。
采用紫外可见光谱、X 射线衍射（XRD）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对合成的 Ag NPs 进行表征。用榕属植物水提物和合成的 Ag NPs 不同浓度处理埃及伊蚊和寒蝉幼虫 24 h，评估其杀幼虫活性。计算半数致死浓度（LC 50）和 r 2 值。
榕属植物粗提物对埃及伊蚊和寒蝉幼虫的最大功效（LC 50=67.72 和 63.70 mg/L；r 2=0.995 和 0.985）和合成的 Ag NPs（LC 50=12.00 和 11.21 mg/L；r 2=0.997 和 0.990）。合成的 Ag NPs 在 2θ 值为 27.61、29.60、35.48、43.48 和 79.68 处出现 XRD 峰，分别鉴定为（210）、（121）、（220）、（200）和（311）反射。Ag NPs 的 FTIR 光谱在 500-3000 cm -1 区域显示出 3425、2878、1627 和 1382 处的显著峰，对应于（NH）C=O 基团的伸缩振动。SEM 分析表明，形状为圆柱形、均匀和棒状，平均尺寸为 250.60nm。
利用榕属植物水提物合成银纳米粒子及其对传播疾病媒介幼虫的杀幼虫活性。合成的 Ag NPs 表现出最大的杀幼虫效果。
