设计一种用于检测霍乱弧菌产毒致病的多重 PCR 方法。

Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae.

机构信息

Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

出版信息

Asian Pac J Trop Med. 2013 Feb;6(2):115-8. doi: 10.1016/S1995-7645(13)60005-X.

DOI:10.1016/S1995-7645(13)60005-X

PMID:23339912

Abstract

OBJECTIVE

To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.

METHODS

Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.

RESULTS

According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.

CONCLUSIONS

Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.

摘要

目的

同时研究霍乱弧菌（V. cholerae）临床株的毒力和调节基因（hlyA、ctxB、tcpI）。

方法

采用聚合酶链反应（PCR）法检测 tcpI、hlyA 和 ctxB 三个重要基因，以检测产毒和致病的 V. cholerae。

结果

根据 PCR 结果，临床分离株中 hlyA、tcpI 和 ctxB 基因的发生率分别为 94.7%（72 例）、90.8%（69 例）和 92.1%（70 例）。五株菌均含有除 ctxB 以外的所有基因，六株菌均含有除 tcpI 以外的所有基因，四株菌均含有除 hlyA 以外的所有基因，一株菌仅含有 hlyA，60 株菌含有包括 hlyA、ctxB 和 tcpI 在内的三个基因的组合。