Rahman Mahbubur, Heng Lee Yook, Futra Dedi, Ling Tan Ling
Department of General Educational Development (GED), Faculty of Science and Information Technology, Daffodil International University, 102 & 102/1, Shukrabad, Mirpur Road, Dhanmondi, Dhaka, 1207, Bangladesh.
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi, 43600 UKM, Selangor D.E., Malaysia.
Nanoscale Res Lett. 2017 Dec;12(1):474. doi: 10.1186/s11671-017-2236-0. Epub 2017 Aug 1.
An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10 to 1.0 × 10 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10 M (R = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.
基于聚苯乙烯 - 丙烯酸（PSA）乳胶纳米球 - 金纳米粒子复合材料（PSA - AuNPs）DNA载体基质，开发了一种用于检测致病性霍乱弧菌（V. cholerae）DNA的超灵敏电化学生物传感器。使用具有电活性蒽醌寡核苷酸标记的差分脉冲伏安法（DPV）来测量生物传感器的响应。金纳米粒子（AuNPs）负载在DNA - 乳胶颗粒电极上显著放大了DNA杂交的法拉第电流。结合使用报道的探针，该生物传感器表现出高灵敏度。该DNA生物传感器对目标DNA的线性响应范围为1.0×10至1.0×10 M，具有可重复性（相对标准偏差，RSD = 4.5%，n = 5），检测限（LOD）为1.0×10 M（R = 0.99）。对于加标样品中霍乱弧菌DNA的检测，该生物传感器获得了91%至109%的满意回收率（n = 3），并且可以连续重复使用六次进行DNA检测，重复性RSD值为5%（n = 5）。在长达58天的储存期内，电化学生物传感器的响应稳定，可维持在其原始响应的95%。
