Nemeth Norbert, Kiss Ferenc, Klarik Zoltan, Peto Katalin, Vanyolos Erzsebet, Toth Laszlo, Furka Istvan, Miko Iren
Department of Operative Techniques and Surgical Research, Institute of Surgery, University of Debrecen, Debrecen, Hungary.
Department of Pathology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Clin Hemorheol Microcirc. 2014;57(3):243-53. doi: 10.3233/CH-131664.
Ischemia-reperfusion-caused hemorheological alterations have been widely studied but the effect of testicular ischemia-reperfusion has not so far. In this study 14 Sprague-Dawley rats were involved. In the ischemia-reperfusion group under general anaesthesia the left testis was explored by opening the scrotum then the deferent duct and vasculature were clamped for 30 minutes. Testicular microcirculation was monitored by laser Doppler flowmetry. The right testis was untouched. In the control group: only anaesthesia was induced. Blood sampling occurred before and after ischemia, at the 60th minute of reperfusion and on the 1st postoperative day for determining hematological parameters (microcell-counter), erythrocyte deformability (slit-flow ektacytometer) and erythrocyte aggregation (light-transmission aggregometer). After the last blood sampling, testicles were removed for histological examination. Hematological parameter changes reflected inflammatory response. Erythrocyte deformability showed a worsening already at the 60th minute of reperfusion compared to base and control values. By the 1st postoperative day further decrease was observed. Erythrocyte aggregation significantly enhanced with great magnitude versus base and control values (p < 0.001). However, conventional histological examinations did not show marked testicular injury. The experienced changes can attract attention to the testicular ischemia-reperfusion causing significant effects on hemorheological parameters, which can lead to further harmful microcirculatory consequences.
缺血再灌注引起的血液流变学改变已得到广泛研究，但睾丸缺血再灌注的影响迄今尚未见报道。本研究纳入了14只Sprague-Dawley大鼠。在缺血再灌注组中，在全身麻醉下打开阴囊暴露左侧睾丸，然后夹闭输精管和血管30分钟。用激光多普勒血流仪监测睾丸微循环。右侧睾丸未作处理。对照组仅进行麻醉诱导。在缺血前后、再灌注第60分钟和术后第1天采集血液样本，以测定血液学参数（血细胞计数器）、红细胞变形性（缝隙流动血细胞变形仪）和红细胞聚集性（透光聚集仪）。最后一次采血后，取出睾丸进行组织学检查。血液学参数变化反映了炎症反应。与基础值和对照组相比，再灌注第60分钟时红细胞变形性就已恶化。到术后第1天，观察到进一步下降。与基础值和对照组相比，红细胞聚集性显著增强（p < 0.001）。然而，传统组织学检查未显示明显的睾丸损伤。所观察到的变化值得关注，即睾丸缺血再灌注对血液流变学参数有显著影响，这可能导致进一步有害的微循环后果。
