Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Hungary.
Clin Hemorheol Microcirc. 2013 Jan 1;53(3):217-29. doi: 10.3233/CH-2012-1539.
Investigation of regional hemorheological properties are important in ischemia-reperfusion experimental surgical and microsurgical research models, however, the physiological arterio-venous as well as porto-caval differences in erythrocyte deformability and aggregation are still controversial. In this study, besides measuring blood pH, blood gases, lactate concentration and hematological parameters we determined erythrocyte deformability (rotational ektacytometer) together with osmoscan data as well as erythrocyte aggregation (light transmittance aggregometer) in blood samples taken from the portal vein, caudal caval vein and abdominal aorta of rats. Blood pH, partial blood gas pressures showed the anticipated physiological differences. Leukocyte count was lower in arterial blood; hematocrit, erythrocyte and platelet count was higher in systemic and portal venous blood. The lowest elongation index values were measured in arterial blood, the highest in venous and portal venous blood showed values in between. The osmoscan data did not show important differences. Erythrocyte aggregation M index 5 s showed the lowest values in arterial, higher in systemic venous and portal venous blood samples. M index 10 s showed significantly low values in systemic venous blood. M1 values were slightly higher in arterial blood compared to venous blood but the highest were in portal venous blood. Erythrocyte deformability and aggregation may show aorto-porto-caval differences in the rat. The appropriate control examinations thus are important in experimental surgical and microsurgical research models.
研究区域血液流变学特性在缺血再灌注实验外科和显微外科研究模型中非常重要，然而，红细胞变形性和聚集的生理动静脉和门腔静脉差异仍然存在争议。在这项研究中，除了测量血液 pH 值、血气、乳酸浓度和血液学参数外，我们还在大鼠的门静脉、尾腔静脉和腹主动脉采集的血液样本中测定了红细胞变形性（旋转 ektacytometer）和渗透压扫描数据以及红细胞聚集（透光率聚集仪）。血液 pH 值、部分血气压力显示出预期的生理差异。白细胞计数在动脉血中较低；血细胞比容、红细胞和血小板计数在全身和门静脉血液中较高。动脉血的伸长指数值最低，静脉和门静脉血的伸长指数值最高，介于两者之间。渗透压扫描数据没有显示出重要差异。红细胞聚集 M 指数 5 s 在动脉血中显示出最低值，在全身静脉和门静脉血样中较高。M 指数 10 s 在全身静脉血中显示出显著的低值。M1 值在动脉血中略高于静脉血，但在门静脉血中最高。红细胞变形性和聚集可能在大鼠中表现出主动脉-门静脉差异。因此，在实验外科和显微外科研究模型中，适当的对照检查非常重要。
