Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Cancer Lett. 2013 Jun 1;333(1):56-65. doi: 10.1016/j.canlet.2013.01.009. Epub 2013 Jan 20.
Cancer stem cells (CSCs) have been implicated in multidrug resistance, a phenomenon responsible for the failure of cancer chemotherapy. However, the underlying mechanisms are still unclear. In our study, we established two oxaliplatin-resistant colorectal cancer cell lines displaying some CSCs characteristics. Oct4 overexpression was observed in these two lines. We performed Oct4 knock down by lentiviral vector-mediated specific shRNA. Knockdown increased apoptosis, decreased CSCs marker expression and weakened tumorigenicity in drug-resistant cell lines. In conclusion, we show that these events can be at least in part attributed to the STAT3/Survivin pathway.
癌症干细胞（CSCs）与多药耐药有关，这是导致癌症化疗失败的一个现象。然而，其潜在的机制尚不清楚。在我们的研究中，我们建立了两种具有某些 CSCs 特征的奥沙利铂耐药结直肠癌细胞系。在这两种细胞系中观察到 Oct4 过表达。我们通过慢病毒载体介导的特异性 shRNA 进行 Oct4 敲低。敲低后，耐药细胞系中的细胞凋亡增加，CSCs 标志物表达减少，致瘤性减弱。总之，我们表明这些事件至少部分归因于 STAT3/Survivin 通路。
