Aebischer M L, Martorana M C, Costa F, Battaggia C, Madera A, Destito D, Machera F, Bailly C, Angeloni P
National Center of Blood Transfusion, Italian Red Cross, Rome, Italy.
Anthropol Anz. 1990 Mar;48(1):15-23.
Microfilter absorbed whole blood samples from 223 Tanzanian babies and 189 adults from Cameroon have been examined. Blood specimens are difficult to obtain from African suburban and rural areas, and lack of storage and transportation facilities can prevent the collection of samples. We evaluated some microassays employing whole blood collected on filter paper. This method is a well established technique in neonatal screening for endocrinometabolic diseases. We also developed microassays for whole dried blood spots to type AB0 blood groups and HIV disease using commercial reagents. Phenotype and gene frequencies for AB0 and hemoglobin systems as well as our results concerning the typings of thyroxine (T4), thyroid stimulating hormone (TSH), human immunodeficiency virus (HIV) and hepatitis B virus (HBV) are reported.
已对从223名坦桑尼亚婴儿以及189名喀麦隆成年人身上采集的微滤吸收全血样本进行了检测。从非洲城郊和农村地区获取血液样本很困难，而且缺乏储存和运输设施会妨碍样本采集。我们评估了一些使用滤纸采集的全血进行的微量分析方法。这种方法在新生儿内分泌代谢疾病筛查中是一种成熟的技术。我们还利用商业试剂开发了用于全干血斑进行ABO血型分型和检测HIV疾病的微量分析方法。报告了ABO和血红蛋白系统的表型及基因频率，以及我们关于甲状腺素（T4）、促甲状腺激素（TSH）、人类免疫缺陷病毒（HIV）和乙型肝炎病毒（HBV）分型的结果。
