Department of Physiology, Zhejiang University School of Medicine, Hangzhou, China.
PLoS One. 2013;8(1):e54563. doi: 10.1371/journal.pone.0054563. Epub 2013 Jan 16.
Nischarin is a protein known to inhibit breast cancer cell motility by regulating the signaling of the Rho GTPase family. However, little is known about its location and function in the nervous system. The aim of the present study was to investigate the regional and cellular expression and functions of Nischarin in the adult rodent brain. As assessed by real-time PCR, Western blot analysis and immunostaining, we found that Nischarin was widely distributed throughout the brain, with a higher expression in the cerebral cortex and hippocampus. Double-labeling showed that Nischarin was expressed in neurons and was mainly located in the perinuclear region and F-actin-rich protrusions. The expression pattern of Nischarin in the brain was thought to be closely associated with its function. This was verified by our findings from cell migration assays that Nischarin regulated neuronal migration. These results provide a preliminary survey of the distribution of Nischarin in different regions and cell types in the rat brain. This might help to elucidate its physiological roles, and to evaluate its potential clinical implications.
Nischarin 是一种已知的蛋白，它通过调节 Rho GTPase 家族的信号来抑制乳腺癌细胞的运动性。然而，关于它在神经系统中的位置和功能知之甚少。本研究旨在探讨 Nischarin 在成年啮齿动物大脑中的区域和细胞表达和功能。通过实时 PCR、Western blot 分析和免疫染色评估，我们发现 Nischarin 在大脑中广泛分布，在大脑皮层和海马体中表达水平更高。双标记显示 Nischarin 在神经元中表达，主要位于核周区域和富含 F-肌动蛋白的突起中。Nischarin 在大脑中的表达模式被认为与其功能密切相关。这一点从我们的细胞迁移实验结果中得到了验证，Nischarin 调节神经元迁移。这些结果初步调查了 Nischarin 在大鼠大脑不同区域和细胞类型中的分布。这可能有助于阐明其生理作用，并评估其潜在的临床意义。
