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富含半胱氨酸的酸性分泌蛋白（SPARC）是人类卵巢癌增殖、凋亡和侵袭的关键调节因子。

SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer.

机构信息

Department of Maternal and Child Health Care, School of Public Health, Shandong University, Jinan, China.

出版信息

PLoS One. 2012;7(8):e42413. doi: 10.1371/journal.pone.0042413. Epub 2012 Aug 3.

DOI:10.1371/journal.pone.0042413
PMID:22879971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3411787/
Abstract

BACKGROUND

Secreted protein acidic and rich in cysteine (SPARC), a calcium-binding matricellular glycoprotein, is implicated in the progression of many cancers. In this study, we investigated the expression and function of SPARC in ovarian cancer.

METHODS

cDNA microarray analysis was performed to compare gene expression profiles of the highly invasive and the low invasive subclones derived from the SKOV3 human ovarian cancer cell line. Immunohistochemistry (IHC) staining was performed to investigate SPARC expression in a total of 140 ovarian tissue specimens. In functional assays, effects of SPARC knockdown on the biological behavior of ovarian cancer cells were investigated. The mechanisms of SPARC in ovarian cancer proliferation, apoptosis and invasion were also researched.

RESULTS

SPARC was overexpressed in the highly invasive subclone compared with the low invasive subclone. High SPARC expression was associated with high stage, low differentiation, lymph node metastasis and poor prognosis of ovarian cancer. Knockdown of SPARC expression significantly suppressed ovarian cancer cell proliferation, induced cell apoptosis and inhibited cell invasion and metastasis.

CONCLUSION

SPARC is overexpressed in highly invasive subclone and ovarian cancer tissues and plays an important role in ovarian cancer growth, apoptosis and metastasis.

摘要

背景

富含半胱氨酸的酸性分泌蛋白（SPARC）是一种钙结合基质细胞糖蛋白，与许多癌症的进展有关。在这项研究中，我们研究了 SPARC 在卵巢癌中的表达和功能。

方法

通过 cDNA 微阵列分析比较了源自 SKOV3 人卵巢癌细胞系的高侵袭性和低侵袭性亚克隆的基因表达谱。通过免疫组织化学（IHC）染色研究了总共 140 个卵巢组织标本中的 SPARC 表达。在功能测定中，研究了 SPARC 敲低对卵巢癌细胞生物学行为的影响。还研究了 SPARC 在卵巢癌增殖、凋亡和侵袭中的作用机制。

结果

与低侵袭性亚克隆相比，SPARC 在高侵袭性亚克隆中过度表达。高 SPARC 表达与卵巢癌的高分期、低分化、淋巴结转移和不良预后相关。SPARC 表达的敲低显著抑制了卵巢癌细胞的增殖，诱导了细胞凋亡，并抑制了细胞侵袭和转移。

结论

SPARC 在高侵袭性亚克隆和卵巢癌组织中过度表达，在卵巢癌的生长、凋亡和转移中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/5a875605b270/pone.0042413.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/bb8ca8108333/pone.0042413.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/de45fe41937d/pone.0042413.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/fdd6ac03e229/pone.0042413.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/e3d56bb11814/pone.0042413.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/fec7f4443c5f/pone.0042413.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/3b88268a5536/pone.0042413.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/5a875605b270/pone.0042413.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/bb8ca8108333/pone.0042413.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/de45fe41937d/pone.0042413.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/fdd6ac03e229/pone.0042413.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/e3d56bb11814/pone.0042413.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/fec7f4443c5f/pone.0042413.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/3b88268a5536/pone.0042413.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e056/3411787/5a875605b270/pone.0042413.g007.jpg

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