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荧光寿命成像显微镜(FLIM)研究活细胞内半导体量子点的摄取和处理。

Uptake and processing of semiconductor quantum dots in living cells studied by fluorescence lifetime imaging microscopy (FLIM).

机构信息

Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada.

出版信息

Chem Commun (Camb). 2013 Feb 28;49(17):1714-6. doi: 10.1039/c3cc36326k.

DOI:10.1039/c3cc36326k
PMID:23344112
Abstract

Carboxylate-terminated and dopamine-conjugated CdSe-ZnS quantum dots (QDs) are imaged in living fibroblasts using fluorescence lifetime imaging microscopy. Changes in lifetime are observed as the QDs are processed in the cells, and are consistent with lifetime measurements in bulk solution using buffers compositions that correspond to different cellular regions.

摘要

采用荧光寿命成像显微镜观察活成纤维细胞中羧酸封端和多巴胺偶联的 CdSe-ZnS 量子点(QDs)。当 QDs 在细胞内被处理时,观察到寿命的变化,这与使用与不同细胞区域相对应的缓冲液组成在体相溶液中进行的寿命测量一致。

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