使用聚合酶链反应（PCR）方法鉴定从人类皮肤利什曼病中分离出的利什曼原虫种类。
Identification of leishmania species isolated from human cutaneous leishmaniasis using PCR method.
作者信息
Rahbarian N, Mesgarian A, Mahmoudi Rad M, Hajaran H, Shahbazi F, Mesgarian Z, Taghipour N
机构信息
Cellular and Molecular Biology Research Center, Shahid Behesthi University, M.C., Tehran, Iran.
出版信息
J Res Health Sci. 2009 Dec 28;9(2):48-51.
BACKGROUND
To determine the epidemiological status of cutaneous leishmaniasis outbreak, isolation and identification of the agent parasite, Leishmania, using PCR method in Gonbad-e Qabus County, north Iran, during 2006-2007.
METHODS
Data were collected on the prevalence of scars and ulcers over a period of 3 months among 6990 inhabitants of five villages around Gonbad-e Qabus County, north Iran, during 2006-2007. Cultured promastigotes were identified using PCR technique. Its1 and its2 of Non Coding Transcribed region at ribosomal DNA of 46 Leishmania isolates were amplified and the PCR products were separated by electrophoresis in 1.5% agarose gel (200 mA, 140 V), visualized by staining with ethidium bromide, and photographed.
RESULTS
Among 6990 inhabitants of 5 villages, 62.9% were identified as scars and 1.5% as active lesions. Individuals 11 to 20 years were the most highly infected age group. All the parasite isolates were Leishmania major.
CONCLUSION
Cutaneous leishmaniasis due to L. major is endemic in Gonbad-e Qabus County, north Iran.
背景
为确定伊朗北部戈纳巴德 - 卡布斯县2006 - 2007年皮肤利什曼病暴发的流行病学状况，采用聚合酶链反应（PCR）方法分离和鉴定病原体寄生虫利什曼原虫。
方法
2006 - 2007年期间，在伊朗北部戈纳巴德 - 卡布斯县周围五个村庄的6990名居民中，收集了为期3个月的疤痕和溃疡患病率数据。使用PCR技术鉴定培养的前鞭毛体。对46株利什曼原虫分离株核糖体DNA非编码转录区的Its1和Its2进行扩增，PCR产物在1.5%琼脂糖凝胶中进行电泳分离（200 mA，140 V），用溴化乙锭染色后观察并拍照。
结果
在5个村庄的6990名居民中，62.9%被鉴定为有疤痕，1.5%为活动性病变。11至20岁的个体是感染率最高的年龄组。所有寄生虫分离株均为硕大利什曼原虫。
结论
伊朗北部戈纳巴德 - 卡布斯县存在由硕大利什曼原虫引起的皮肤利什曼病地方性流行。
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