Tolouei Sepideh, Hejazi Seyed Hossein, Ghaedi Kamran, Hasheminia Seyed Javad
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Department of Biology, School of Sciences, University of Isfahan, Isfahan, IR Iran.
Jundishapur J Microbiol. 2014 Apr;7(4):e9529. doi: 10.5812/jjm.9529. Epub 2014 Apr 1.
Cutaneous Leishmaniasis (CL) is a parasitic disease in most parts of Iran, especially in the Isfahan province. The most common form of CL is a self-healing lesion but in rare situations, infection might develop to non-healing forms. Clinical symptoms and treatment process might be influenced by several agents such as host immune response and parasite strains. In this study, the isolates which caused healing and nonhealing forms of CL in Isfahan were characterized by internal transcribed spacer (ITS) polymerase chain reaction (PCR) technique.
The aim of this study was to identify Leishmania species isolated from healing and non-healing CLs using PCR method.
Thirty patients resident in Isfahan province, with healing or non-healing form of CL were entered into this study. After DNA extraction, the identification of Leishmania isolates was done by ITS1-PCR method.
Leishmania major was found as the predominant species (100%) in both healing and non-healing forms of CL.
It seems that there is no difference between Leishmania species in healing and non-healing forms of CL. In order to recognize the reason of long lasting lesions in non-healing patients, the study about parasite strains and immune factors at the molecular level mostly in nonhealing patient is recommended.
皮肤利什曼病（CL）在伊朗大部分地区，尤其是伊斯法罕省，是一种寄生虫病。CL最常见的形式是可自愈的损伤，但在罕见情况下，感染可能发展为不愈合形式。临床症状和治疗过程可能受多种因素影响，如宿主免疫反应和寄生虫菌株。在本研究中，采用内转录间隔区（ITS）聚合酶链反应（PCR）技术对伊斯法罕引起CL愈合和不愈合形式的分离株进行了鉴定。
本研究旨在使用PCR方法鉴定从愈合和不愈合的CL中分离出的利什曼原虫种类。
30名居住在伊斯法罕省、患有愈合或不愈合形式CL的患者纳入本研究。DNA提取后，通过ITS1-PCR方法对利什曼原虫分离株进行鉴定。
在CL的愈合和不愈合形式中，均发现硕大利什曼原虫为主要种类（100%）。
CL的愈合和不愈合形式中利什曼原虫种类似乎没有差异。为了认识不愈合患者长期损伤的原因，建议主要针对不愈合患者在分子水平上开展关于寄生虫菌株和免疫因素的研究。
