Oncology Department of Internal Medicine, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China.
Molecules. 2013 Jan 11;18(1):845-58. doi: 10.3390/molecules18010845.
In this study, the anticancer activity of chamaejasmine was characterized in the human breast cancer cell line, MDA-MB-231. Cell viability and cell cycle distribution were determined by MTT assay and flow cytometry, respectively. Western blotting was performed to determine changes in levels of various proteins. Results showed that treatment with chamaejasmine (4-16 μM) inhibited cell proliferation, which correlated with G2/M phase arrest and apoptosis in MDA-MB-231 cells. Chamaejasmine treatment of MDA-MB-231 cells resulted in induction of WAF1/p21 and KIP1/p27, decrease in cyclins A and cyclins B1. Cyclin-dependent kinase (cdk) 2 and cdc2 was also decreased after chamaejasmine treatment. Moreover, inhibition of nuclear translocation, phosphorylation of NF-κB, activation of IKKα and IKKβ, inhibition of phosphorylation and degradation of IκBα were also detected in this work. Our findings suggested that chamaejasmine could be explored as a preventive and perhaps as a chemotherapeutic agent in the management of breast cancer.
在这项研究中，我们研究了香叶树素在人乳腺癌细胞系 MDA-MB-231 中的抗癌活性。通过 MTT 检测法和流式细胞术分别测定细胞活力和细胞周期分布。通过 Western blot 测定各种蛋白水平的变化。结果表明，香叶树素（4-16 μM）处理抑制细胞增殖，与 MDA-MB-231 细胞的 G2/M 期阻滞和细胞凋亡相关。香叶树素处理 MDA-MB-231 细胞导致 WAF1/p21 和 KIP1/p27 的诱导，细胞周期蛋白 A 和细胞周期蛋白 B1 的减少。香叶树素处理后细胞周期蛋白依赖性激酶（cdk）2 和 cdc2 也减少。此外，本工作还检测到核易位、NF-κB 磷酸化、IKKα 和 IKKβ 的激活、磷酸化和 IκBα 降解的抑制。我们的研究结果表明，香叶树素可以作为预防和治疗乳腺癌的潜在药物进行探索。
