School of Life Science, Sun Yat-sen University, Guangzhou 510275, China.
Pharm Res. 2010 Jun;27(6):1128-37. doi: 10.1007/s11095-010-0052-7. Epub 2010 Mar 12.
We evaluated the chemotherapeutic effect of water extract of white cocoa tea (WCTE) against human prostate cancer (PCa) in vitro and in vivo.
Cell viability and cell cycle distribution were determined by MTT assay and flow cytometry, respectively. Western blotting was performed to determine changes in levels of various proteins. Effect of WCTE was determined in athymic nude mice implanted with PC-3 cells.
Treatment with WCTE (100-150 microg/ml) inhibited cell proliferation, which correlated with G2/M phase arrest in PC-3 cells. WCTE treatment to PC-3 cells resulted in (1) induction of WAF1/p21 and KIP1/p27, (2) decrease in cyclins D1, D2 and E, (3) decrease in cyclin-dependent kinase (cdk) 2, 4 and 6, (4) induction of Bax and down-regulation of Bcl-2, (5) decrease in procaspase-3, -8, (6) inhibition of nuclear translocation and phosphorylation of NF-kappaB and activation of IKKalpha, and (7) inhibition of phosphorylation and degradation of IkappaBalpha. Oral administration of WCTE (0.1 and 0.2%, wt/vol) to athymic nude mice resulted in greater than 50% inhibition of tumor growth. There was a decrease in expressions of cyclin D1, Bcl-2 and p-NF-kappaB and an increase in WAF1/p21 and Bax in tumor tissues of mice.
WCTE can be a useful chemotherapeutic agent against human PCa.
我们评估了白可可茶（WCTE）水提取物对体外和体内人前列腺癌（PCa）的化疗作用。
通过 MTT 测定法和流式细胞术分别测定细胞活力和细胞周期分布。通过 Western blot 测定来确定各种蛋白质水平的变化。在植入 PC-3 细胞的裸鼠中确定 WCTE 的作用。
用 WCTE（100-150μg/ml）处理可抑制 PC-3 细胞的增殖，这与 G2/M 期阻滞相关。WCTE 处理 PC-3 细胞导致：（1）WAF1/p21 和 KIP1/p27 的诱导；（2）细胞周期蛋白 D1、D2 和 E 的减少；（3）细胞周期蛋白依赖性激酶（cdk）2、4 和 6 的减少；（4）Bax 的诱导和 Bcl-2 的下调；（5）procaspase-3、-8 的减少；（6）NF-κB 的核转位和磷酸化的抑制以及 IKKα 的激活；（7）IkappaBα 的磷酸化和降解的抑制。WCTE（0.1 和 0.2%，wt/vol）的口服给药导致裸鼠肿瘤生长抑制大于 50%。在小鼠肿瘤组织中，cyclin D1、Bcl-2 和 p-NF-κB 的表达减少，而 WAF1/p21 和 Bax 的表达增加。
WCTE 可能是一种治疗人前列腺癌的有用化疗药物。
