Department of Oncology, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
Molecules. 2013 Jan 11;18(1):934-50. doi: 10.3390/molecules18010934.
Metastasis is the major cause of cancer-related deaths. Targeting the process of metastasis has been proposed as a strategy to fight cancer. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is used for treatment of many diseases, including cancer. This study aimed to determine the anti-metastatic effect of total flavonoids of S. barbata (TF-SB) using the human hepatocarcinoma MHCC97H cell line with high metastatic potential. Our results show that TF-SB could significantly inhibit the proliferation and invasion of MHCC97H cells in a dose-dependent manner. MMP-2 and MMP-9 expression were obviously decreased after TF-SB treatment at both the mRNA and protein level. TIMP-1 and TIMP-2 expression were simultaneously increased. The present study indicates that TF-SB could reduce the metastatic capability of MHCC97H cell, probably through decrease of the MMP expression, and simultaneous increase of the TIMP expression.
转移是癌症相关死亡的主要原因。针对转移过程已被提议作为一种抗癌策略。黄芩（Scutellaria barbata D. Don）是一种传统中药，用于治疗多种疾病，包括癌症。本研究旨在使用具有高转移潜能的人肝癌 MHCC97H 细胞系来确定黄芩总黄酮（TF-SB）的抗转移作用。我们的结果表明，TF-SB 可显著抑制 MHCC97H 细胞的增殖和侵袭，呈剂量依赖性。TF-SB 处理后 MMP-2 和 MMP-9 的表达在 mRNA 和蛋白水平上均明显降低。TIMP-1 和 TIMP-2 的表达同时增加。本研究表明，TF-SB 可能通过降低 MMP 的表达，同时增加 TIMP 的表达，从而降低 MHCC97H 细胞的转移能力。
