Zuhur Sayid Shafi, Tanik Canan, Erol Rumeysa Selvinaz, Musluman Ahmet Murat, Kabukcuoglu Fevziye, Altuntas Yuksel
Sisli Etfal Training and Research Hospital, Department of Endocrinology and Metabolism, Istanbul, Turkey.
Turk Neurosurg. 2013;23(1):55-60. doi: 10.5137/1019-5149.JTN.6706-12.1.
To determine the immunohistochemical expression of ErbB2 in adamantinomatous craniopharyngiomas (ACP) and to assess its relationship with nuclear expression of β-catenin in surgically resected human ACP tissue sections and to estimate whether these tumors could be candidates for anti-ErbB2 therapy.
The ErbB2 and β-catenin immunostaining was performed on paraffin embedded tissue sections of 20 ACP using avidin-biotin-peroxidase complex method. ErbB2 immunoreactivity was interpreted according to the American Society of Clinical Oncology/ College of American Pathologists criterions for breast carcinoma.
Foci of nuclear reactivity for β-catenin was observed in all ACP tissue specimens mainly concentrated in whorl like arrays of the epithelial cells. Two (10%) of the cases were score 3+ for ErbB2 as demonstrated by strong complete membrane staining. However, the localization of 3+ ErbB2 cells was different from those with nuclear β-catenin immunoreactivity.
Our preliminary data demonstrate score 3+ staining for ErbB2 in 10% of ACP and different localization of 3+ ErbB2 cells and cells with nuclear β-catenin immunoreactivity. However, because of the small number of cases, further studies with larger samples should be conducted to verify and validate our preliminary data and to determine the effect of ErbB2 protein in ACP cell growth, survival and differentiation.
确定埃布B2（ErbB2）在造釉细胞瘤型颅咽管瘤（ACP）中的免疫组化表达，评估其与手术切除的人ACP组织切片中β-连环蛋白核表达的关系，并评估这些肿瘤是否可能是抗ErbB2治疗的候选对象。
采用抗生物素蛋白-生物素-过氧化物酶复合物法，对20例ACP石蜡包埋组织切片进行ErbB2和β-连环蛋白免疫染色。根据美国临床肿瘤学会/美国病理学家协会乳腺癌标准解释ErbB2免疫反应性。
在所有ACP组织标本中均观察到β-连环蛋白核反应灶，主要集中在上皮细胞的漩涡状排列中。2例（10%）病例的ErbB2评分为3+，表现为强烈的完全膜染色。然而，3+ ErbB2细胞的定位与核β-连环蛋白免疫反应性细胞不同。
我们的初步数据显示，10%的ACP中ErbB2染色为3+，且3+ ErbB2细胞与核β-连环蛋白免疫反应性细胞的定位不同。然而，由于病例数量较少，应进行更大样本量的进一步研究，以验证和确认我们的初步数据，并确定ErbB2蛋白在ACP细胞生长、存活和分化中的作用。
