Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
J Fluoresc. 2013 May;23(3):375-9. doi: 10.1007/s10895-013-1161-6. Epub 2013 Jan 24.
A protein-based emission ratiometric fluorescence biosensor is described that exhibits sensitivity to free zinc ion in solution down to picomolar concentrations. Ratiometric measurements are widely used to assure accurate quantitation, and emission ratios are preferred for laser scanning microscopes such as confocal fluorescence microscopes. The relatively long emission wavelengths used are well suited to studies in tissues and other matrices which exhibit significant fluorescence background, and the apo-carbonic anhydrase moiety recognizes zinc ion with high and controllable specificity.
本文描述了一种基于蛋白质的发射比率荧光生物传感器，它对溶液中的游离锌离子具有灵敏度，可检测至皮摩尔浓度。比率测量被广泛用于确保准确的定量，并且发射比率被优先用于激光扫描显微镜，如共聚焦荧光显微镜。所使用的相对较长的发射波长非常适合于在组织和其他基质中进行研究，这些基质表现出显著的荧光背景，并且脱辅基碳酸酐酶部分对锌离子具有高可控特异性的识别。
