Kamzolova S G, Ivanova N N, Kamzalov S S
Institute of Cell Biophysics of Russian Academy of Sciences, Pushchino, Moscow region, 142292 Russia.
J Biol Phys. 1999 Jun;24(2-4):157-66. doi: 10.1023/A:1005135800715.
Conformation behavior of phase T2 DNA in the process of its interaction with it E. coli RNA polymerase was studied using spin labeling technique. T2 DNA was modified by the spin-labeled imidazole at OH-groups of glucosylated cytidine residues. It was shown that the binding of RNA polymerase under the conditions favoring the formation of open promoter complexes induces specific conformational changes in the spin-labeled DNA. The observed conformational changes encompass not only the promoter regions of DNA which are involved in direct contacts with RNA polymerase molecules but extend over remote DNA sites (long-range effect). In relation to this effect, current theoretical models of DNA dynamics are discussed.
利用自旋标记技术研究了T2期DNA与大肠杆菌RNA聚合酶相互作用过程中的构象行为。通过自旋标记的咪唑对糖基化胞嘧啶残基的羟基进行修饰，从而对T2 DNA进行了改性。结果表明，在有利于形成开放启动子复合物的条件下，RNA聚合酶的结合会诱导自旋标记DNA发生特定的构象变化。观察到的构象变化不仅包括与RNA聚合酶分子直接接触的DNA启动子区域，还延伸到远程DNA位点（远距离效应）。针对这种效应，讨论了当前DNA动力学的理论模型。
