[通过自旋标记对T4 DNA中胞苷进行特定化学修饰]
[Specific chemical modification of cytidine in T4 DNA by a spin label].
作者信息
Kamzolova S G
出版信息
Biokhimiia. 1987 Sep;52(9):1577-82.
PMID:2823914
Abstract
A procedure for selective modification of DNA from T4 phage non-glucosylated mutant by the spin label--N(2,2',5,5') tetramethyl-3-carboxypyrrolidine-1-oxyl)-imidazole was developed. The spin label was shown to interact with hydroxyl groups of 5-hydroxymethyl-2 deoxycytidines. The modification does not affect the secondary structure of DNA, its conformation or template properties in a cell-free system of RNA synthesis.
摘要
开发了一种通过自旋标记物——N(2,2',5,5')-四甲基-3-羧基吡咯烷-1-氧基)-咪唑对T4噬菌体非糖基化突变体的DNA进行选择性修饰的方法。结果表明，该自旋标记物可与5-羟甲基-2'-脱氧胞苷的羟基相互作用。在无细胞RNA合成系统中，这种修饰不会影响DNA的二级结构、构象或模板特性。
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