MX（3-氯-4-（二氯甲基）-5-羟基-2（5H）-呋喃酮）处理的胶原蛋白直流电导率的临时变化
Temporary Changes in the dc ElectricalConductivity of MX (3-Chloro-4(Dichloromethyl)-5-Hydroxy-2(5H)-Furanone) Treated Collagen.
作者信息
Kubisz L, Andrzejewski P
出版信息
J Biol Phys. 2001 Dec;27(4):285-94. doi: 10.1023/A:1014298301405.
The influence of MX(3-Chloro-4(Dichloromethyl)-5-Hydroxy-2(5H)- Furanone), a stronglymutagenic compound, on the temperature dependence of the dcelectrical conductivity of collagen as a function of time was studied.Collagen was immersed in MX solution, next dried and pressed intotablets. The MX concentration was measured by HPLC analysis.The reduction of MX concentration to 10% of the initial value wasobserved in the presence of collagen in the solution, whereas in thecontrol solution concentration of MX decreased to 70% of the initialvalue. Measurements of electrical conductivity were performed for thetemperature range 295-453K and activation energies for the chargeconduction process were calculated. Within the temperature range295-340K, the presence of MX decreased electrical conductivity ofcollagen. Calculated activation energies were typical for dry proteins.Within the temperature range 295-320K activation energy decreasedwith time, probably due to the stronger interactions in thecollagen-water-MX system. For temperatures between 320-410 and430-450K the activation energy was not time dependent and theapplication of MX did not change the structure of the collagenmacromolecule. The temporary changes occurring at the lowertemperatures being due solely to changes in the collagen-waterinteractions.
研究了强诱变化合物MX（3-氯-4-（二氯甲基）-5-羟基-2（5H）-呋喃酮）对胶原蛋白直流电导率随时间变化的温度依赖性的影响。将胶原蛋白浸入MX溶液中，然后干燥并压制成片剂。通过高效液相色谱分析测量MX浓度。在溶液中存在胶原蛋白的情况下，观察到MX浓度降低至初始值的10%，而在对照溶液中，MX浓度降低至初始值的70%。在295-453K的温度范围内进行电导率测量，并计算电荷传导过程的活化能。在295-340K的温度范围内，MX的存在降低了胶原蛋白的电导率。计算出的活化能是干燥蛋白质的典型值。在295-320K的温度范围内，活化能随时间降低，这可能是由于胶原蛋白-水-MX系统中更强的相互作用。对于320-410K和430-450K之间的温度，活化能与时间无关，并且MX的应用没有改变胶原蛋白大分子的结构。在较低温度下发生的暂时变化仅归因于胶原蛋白-水相互作用的变化。
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