控制和测量脂质双分子层膜中的局部组成和性质。
Controlling and measuring local composition and properties in lipid bilayer membranes.
作者信息
D'Onofrio T G, Binns C W, Muth E H, Keating C D, Weiss P S
机构信息
Department of Chemistry, The Pennsylvania State University, University Park, PA 16802 USA.
出版信息
J Biol Phys. 2002 Dec;28(4):605-17. doi: 10.1023/A:1021278420558.
Abstract
Local composition, structure, morphology, and phase are interrelated in lipid bilayer membranes. This gives us the opportunity to control one or more of these properties by manipulating others. We investigate theserelationships with combinations of simultaneous two-color widefield fluorescence imaging, three-dimensional rendering of vesicle domains, andmanipulation of the vesicle morphology via optical trapping and micropipetteaspiration. We describe methods to modulate, to measure, and to probe thelocal structure of model membranes through control of membrane curvature inliposomes.
摘要
脂质双分子层膜中的局部组成、结构、形态和相是相互关联的。这使我们有机会通过操纵其他特性来控制其中一个或多个特性。我们通过同时进行双色宽场荧光成像、囊泡域的三维渲染以及通过光镊和微吸管抽吸操纵囊泡形态的组合来研究这些关系。我们描述了通过控制脂质体中的膜曲率来调节、测量和探测模型膜局部结构的方法。
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