Pasciak P, Krawczyk M J, Gudowska-Nowak E, Kulakowski K
Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30-059 Kraków, Poland.
J Biol Phys. 2005 Dec;31(3-4):365-73. doi: 10.1007/s10867-005-7287-2.
Problems related to the gel electrophoretic migration of polymers can be investigated by models based on a Brownian-type ratchet where a particle can undergo a net transport on a potential energy surface that is externally driven to fluctuate between several distinct states. Here we describe the method of polymer transport and separation by means of the cellular automata technique. Numerical simulations of the polymer reptation in the model system allow us to understand the band-broadening processes in the gel electrophoresis experiments. They indicate also possible ways of fine-tuning the parameters in designing desired resolution of the experiments.
与聚合物凝胶电泳迁移相关的问题可以通过基于布朗型棘轮的模型来研究，在该模型中，粒子可以在外部驱动下在几个不同状态之间波动的势能面上进行净传输。在这里，我们描述了利用细胞自动机技术进行聚合物传输和分离的方法。对模型系统中聚合物爬行的数值模拟使我们能够理解凝胶电泳实验中的谱带展宽过程。它们还指出了在设计所需实验分辨率时微调参数的可能方法。
