Amigo Cristina Román, Sena de Gobbi Debora Dirani, Gomes Vasco Túlio de Moura, Perina Danilo do Prado, Nogueira de Lima Filsner Pedro Henrique, Costa Barbara Letícia Pereira, Spindola Maria Garcia, Ferreira Thais Sebastiana Porfida, Brandão Paulo Eduardo, Moreno Andrea Micke
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Avenida Professor Dr. Orlando Marques de Paiva 87 Cidade Universitária, 05508 270 São Paulo, SP, Brazil.
ScientificWorldJournal. 2012;2012:572732. doi: 10.1100/2012/572732. Epub 2012 Dec 30.
Actinobaculum suis is an important agent related to urinary infection in swine females. Due to its fastidious growth characteristics, the isolation of this anaerobic bacterium is difficult, thus impairing the estimation of its prevalence. The purpose of this study was to develop and test a polymerase chain reaction (PCR) for the detection and identification of A. suis and then compare these results with traditional isolation methods. Bacterial isolation and PCR were performed on one hundred and ninety-two urine samples from sows and forty-five preputial swabs from boars. The results indicate that this PCR was specific for A. suis, presenting a detection limit between 1.0 × 10(1) CFU/mL and 1.0 × 10(2) CFU/mL. A. suis frequencies, as measured by PCR, were 8.9% (17/192) in sow urine samples and 82.2% (37/45) in preputial swabs. Assessed using conventional culturing techniques, none of the urine samples were positive for A. suis; however, A. suis was detected in 31.1% (14/45) of the swabs. This PCR technique was shown to be an efficient method for the detection of A. suis in urine and preputial swabs.
猪放线杆菌是与母猪泌尿系统感染相关的一种重要病原体。由于其苛求的生长特性，分离这种厌氧菌很困难，从而影响了对其流行情况的评估。本研究的目的是开发并测试一种用于检测和鉴定猪放线杆菌的聚合酶链反应（PCR），然后将这些结果与传统分离方法进行比较。对来自母猪的192份尿液样本和来自公猪的45份包皮拭子进行了细菌分离和PCR检测。结果表明，该PCR对猪放线杆菌具有特异性，检测限在1.0×10¹ CFU/mL至1.0×10² CFU/mL之间。通过PCR检测，母猪尿液样本中猪放线杆菌的检出率为8.9%（17/192），包皮拭子中为82.2%（37/45）。采用传统培养技术评估，尿液样本中均未检测到猪放线杆菌阳性；然而，在31.1%（14/45）的拭子中检测到了猪放线杆菌。该PCR技术被证明是检测尿液和包皮拭子中猪放线杆菌的有效方法。
