de Wolf J T, Beentjes J A, Esselink M T, Smit J W, Halie M R, Vellenga E
Department of Medicine, University of Groningen, The Netherlands.
Br J Haematol. 1990 Mar;74(3):246-50. doi: 10.1111/j.1365-2141.1990.tb02578.x.
Human recombinant interleukin 4 (IL-4) was studied for its effects on the erythroid burst forming unit (BFU-E) from human bone marrow cells. IL-4 alone neither supports nor suppresses the erythropoietin (Epo)-dependent colony formation. Different results were obtained when IL-4 was combined with interleukin-3 (IL-3) in the presence of Epo. IL-4 suppressed the IL-3 supported erythroid colony formation in all cases (an increase of 58 +/- 8% with IL-3 versus an increase of 14 +/- 7% with IL-3 plus IL-4, n = 8). This antagonizing effect was dependent on the continuous presence of IL-4 in the culture medium, but was independent of adherent cells, B-, T-cells, or the presence of serum in the culture medium. Finally, the effects of IL-4 and IL-3 were studied on the 'Epo-independent' BFU-E by adding Epo on day 3. A decline of the IL-3 supported BFU-E was observed in the presence of IL-4 but the degree of reduction was equivalent to the results obtained when Epo was supplied at day 0. These findings indicate that IL-4 acts as suppressive growth factor for the IL-3 supported erythroid colony formation from human bone marrow cells.
研究了重组人白细胞介素4（IL-4）对人骨髓细胞红系爆式集落形成单位（BFU-E）的影响。单独使用IL-4既不支持也不抑制促红细胞生成素（Epo）依赖的集落形成。当在Epo存在的情况下将IL-4与白细胞介素-3（IL-3）联合使用时，得到了不同的结果。在所有情况下，IL-4均抑制IL-3支持的红系集落形成（IL-3单独作用时集落增加58±8%，而IL-3加IL-4时增加14±7%，n = 8）。这种拮抗作用取决于培养基中持续存在IL-4，但与贴壁细胞、B细胞、T细胞或培养基中血清的存在无关。最后，通过在第3天添加Epo，研究了IL-4和IL-3对“Epo非依赖型”BFU-E的影响。在IL-4存在的情况下观察到IL-3支持的BFU-E数量下降，但减少程度与第0天提供Epo时获得的结果相当。这些发现表明，IL-4对IL-3支持的人骨髓细胞红系集落形成起抑制生长因子的作用。
