Lewis C G, Fields M, Beal T
Carbohydrate Nutrition Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Br J Nutr. 1990 Mar;63(2):387-95. doi: 10.1079/bjn19900124.
The present study was designed to examine the effects of various levels of fructose in a copper-deficient diet on some of the signs of Cu deficiency in the rat. Weanling male rats were randomly assigned to one of five diets which contained 0.6 micrograms Cu/g diet and 627 g carbohydrate/kg which was (g/kg): 627 fructose (diet 100); 470 fructose, 157 starch (diet 75); 313.5 fructose, 313.5 starch (diet 50); 157 fructose, 470 starch (diet 25); or 627 starch (diet 0). Rats ate their respective diets for either 2 or 5 weeks. There was a significant linear inverse response of body-weight (P less than 0.0001), packed cell volume (P less than 0.0001) and erythrocyte superoxide dismutase (EC 1.15.1.1) activity (P less than 0.008) to increasing levels of dietary fructose and a direct linear response of plasma cholesterol (P less than 0.05) and blood urea nitrogen concentrations (P less than 0.001) to increasing levels of dietary fructose. Liver, kidney and pancreatic Cu concentrations decreased in a dose-response manner as the level of dietary fructose increased. In general, if fructose was included in the diet the signs of Cu deficiency were exacerbated in a dose-response manner.
本研究旨在检测铜缺乏饮食中不同水平的果糖对大鼠某些铜缺乏症状的影响。将断乳雄性大鼠随机分为五组，每组大鼠分别喂食一种饮食，这些饮食含0.6微克铜/克食物，627克碳水化合物/千克，其中碳水化合物的组成（克/千克）分别为：627果糖（饮食100）；470果糖、157淀粉（饮食75）；313.5果糖、313.5淀粉（饮食50）；157果糖、470淀粉（饮食25）；或627淀粉（饮食0）。大鼠分别食用各自的饮食2周或5周。随着饮食中果糖水平的升高，体重（P<0.0001）、红细胞压积（P<0.0001）和红细胞超氧化物歧化酶（EC 1.15.1.1）活性（P<0.008）呈显著线性负反应，而血浆胆固醇（P<0.05）和血尿素氮浓度（P<0.001）呈显著线性正反应。随着饮食中果糖水平的升高，肝脏、肾脏和胰腺中的铜浓度呈剂量反应性降低。一般来说，如果饮食中含有果糖，铜缺乏的症状会以剂量反应的方式加剧。
