Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, Saga 840-8502, Japan.
J Phys Chem B. 2013 Feb 7;117(5):1321-5. doi: 10.1021/jp4001187. Epub 2013 Jan 24.
We have developed a near-infrared excited Raman optical activity (ROA) spectrometer and report the first measurement of near-infrared ROA spectra of a light-driven proton pump, bacteriorhodopsin. Our results demonstrate that a near-infrared excitation enables us to measure the ROA spectra of the chromophore within a protein environment. Furthermore, the ROA spectra of the all-trans, 15-anti and 13-cis, 15-syn isomers differ significantly, indicating a high structural sensitivity of the ROA spectra. We therefore expect that future applications of the near-infrared ROA will allow the experimental elucidation of the active site structures in other proteins as well as reaction intermediates.
我们开发了一种近红外激发拉曼光学活性（ROA）光谱仪，并报告了首例对光驱动质子泵细菌视紫红质的近红外 ROA 光谱的测量。我们的结果表明，近红外激发使我们能够测量蛋白质环境中发色团的 ROA 光谱。此外，全反式、15-反式和 13-顺式、15-顺式异构体的 ROA 光谱差异显著，表明 ROA 光谱具有很高的结构灵敏度。因此，我们预计近红外 ROA 的未来应用将能够实验阐明其他蛋白质中的活性位点结构以及反应中间体。
