Department of Pathology, Department of Medicine, Center for Transfusion Medicine Research, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; Geisel School of Medicine at Dartmouth, Hanover, New Hampshire; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota.
Transfusion. 2013 Oct;53(10):2307-13. doi: 10.1111/trf.12083. Epub 2013 Jan 24.
The platelet (PLT) Pan Genera Detection test (PGD) is a rapid bacterial detection system used to screen PLTs for bacterial contamination. We report a single center 46-month experience with secondary screening of apheresis PLTs by PGD testing.
Existing testing records of apheresis PLTs screened by PGD from July 2008 to April 2012 were reviewed. All PLT units were initially screened by routine postcollection culture methods. Secondary screening using PGD was performed for indated PLTs on PLT storage Day 4 and for outdated PLTs on Day 8.
A total of 8535 apheresis PLTs were available in inventory during the study period. Of these, 5030 (58.9%) were dispensed and transfused before PGD testing and 3505 (41.1%) underwent PGD testing on Day 4. Twenty-five units tested on Day 4 were PGD initial reactive (0.71%). All were confirmed to be false positive by repeat PGD testing in triplicate (n=20) or by confirmatory culture (n=5). An additional 364 units that were PGD nonreactive on Day 4 were approved for transfusion on Day 6 or Day 7 due to urgent clinical need. A total of 371 outdated units underwent repeat PGD testing before discard on Day 8; all were nonreactive.
Secondary PGD testing of culture-screened apheresis PLTs results in low yield in a medium-sized transfusion service. Use of PGD testing on Day 4 may allow for extension of the apheresis PLT shelf life to Day 7 for hospitals that face supply constraints.
血小板（PLT）泛属检测试验（PGD）是一种快速细菌检测系统，用于筛选血小板是否受到细菌污染。我们报告了一家单中心 46 个月的经验，即通过 PGD 检测对单采血小板进行二次筛选。
回顾了 2008 年 7 月至 2012 年 4 月期间通过 PGD 筛选的单采血小板的现有检测记录。所有血小板单位最初均通过常规采集后培养方法进行筛选。对过期的血小板在储存第 4 天和第 8 天进行 PGD 二次筛选。
在研究期间，库存中共有 8535 单位单采血小板。其中，5030 单位（58.9%）在进行 PGD 检测之前已发放和输注，3505 单位（41.1%）在第 4 天进行了 PGD 检测。第 4 天检测到的 25 个单位 PGD 初筛呈阳性（0.71%）。所有单位经 PGD 重复检测（n=20）或确认培养（n=5）均证实为假阳性。由于临床急需，364 个在第 4 天 PGD 检测呈阴性的单位在第 6 天或第 7 天获得批准输注。在第 8 天丢弃之前，对总共 371 个过期单位进行了重复 PGD 检测，结果均为阴性。
在中等规模的输血服务中，对培养筛选的单采血小板进行二次 PGD 检测的产量较低。对于面临供应限制的医院，在第 4 天使用 PGD 检测可能会将单采血小板的保质期延长至第 7 天。
